6VWT

Transitional unit cell 1 of adenine riboswitch aptamer crystal phase transition upon ligand binding

6VWT の概要

• エントリーDOI: 10.2210/pdb6vwt/pdb
• 関連するPDBエントリー: 5E54
• 分子名称: adenine riboswitch aptamer variant, ADENINE, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: riboswitch, phase transition, time-resolved, rna
• 由来する生物種: Vibrio vulnificus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 45839.46

構造登録者

Stagno, J.R.,Wang, Y.-X. (登録日: 2020-02-20, 公開日: 2020-11-11, 最終更新日: 2023-10-11)

主引用文献

Ramakrishnan, S.,Stagno, J.R.,Conrad, C.E.,Ding, J.,Yu, P.,Bhandari, Y.R.,Lee, Y.T.,Pauly, G.,Yefanov, O.,Wiedorn, M.O.,Knoska, J.,Oberthur, D.,White, T.A.,Barty, A.,Mariani, V.,Li, C.,Brehm, W.,Heinz, W.F.,Magidson, V.,Lockett, S.,Hunter, M.S.,Boutet, S.,Zatsepin, N.A.,Zuo, X.,Grant, T.D.,Pandey, S.,Schmidt, M.,Spence, J.C.H.,Chapman, H.N.,Wang, Y.X.
Synchronous RNA conformational changes trigger ordered phase transitions in crystals.
Nat Commun, 12:1762-1762, 2021

PubMed Abstract: Time-resolved studies of biomacromolecular crystals have been limited to systems involving only minute conformational changes within the same lattice. Ligand-induced changes greater than several angstroms, however, are likely to result in solid-solid phase transitions, which require a detailed understanding of the mechanistic interplay between conformational and lattice transitions. Here we report the synchronous behavior of the adenine riboswitch aptamer RNA in crystal during ligand-triggered isothermal phase transitions. Direct visualization using polarized video microscopy and atomic force microscopy shows that the RNA molecules undergo cooperative rearrangements that maintain lattice order, whose cell parameters change distinctly as a function of time. The bulk lattice order throughout the transition is further supported by time-resolved diffraction data from crystals using an X-ray free electron laser. The synchronous molecular rearrangements in crystal provide the physical basis for studying large conformational changes using time-resolved crystallography and micro/nanocrystals.

PubMed: 33741910
DOI: 10.1038/s41467-021-21838-5

実験手法

X-RAY DIFFRACTION (3.03 Å)