6VVJ

Cap1G-TPUA

6VVJ の概要

• エントリーDOI: 10.2210/pdb6vvj/pdb
• NMR情報: BMRB: 30724
• 分子名称: RNA (130-MER) (1 entity in total)
• 機能のキーワード: rna, capped rna, viral, mal strain, mal, 5' leader, leader rna, covered cap
• 由来する生物種: Human immunodeficiency virus 1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 41922.79

構造登録者

Summers, M.F.,Brown, J.D. (登録日: 2020-02-18, 公開日: 2020-04-29, 最終更新日: 2024-05-15)

主引用文献

Brown, J.D.,Kharytonchyk, S.,Chaudry, I.,Iyer, A.S.,Carter, H.,Becker, G.,Desai, Y.,Glang, L.,Choi, S.H.,Singh, K.,Lopresti, M.W.,Orellana, M.,Rodriguez, T.,Oboh, U.,Hijji, J.,Ghinger, F.G.,Stewart, K.,Francis, D.,Edwards, B.,Chen, P.,Case, D.A.,Telesnitsky, A.,Summers, M.F.
Structural basis for transcriptional start site control of HIV-1 RNA fate.
Science, 368:413-417, 2020

PubMed Abstract: Heterogeneous transcriptional start site usage by HIV-1 produces 5'-capped RNAs beginning with one, two, or three 5'-guanosines (1G, 2G, or 3G, respectively) that are either selected for packaging as genomes (1G) or retained in cells as translatable messenger RNAs (mRNAs) (2G and 3G). To understand how 5'-guanosine number influences fate, we probed the structures of capped HIV-1 leader RNAs by deuterium-edited nuclear magnetic resonance. The 1G transcript adopts a dimeric multihairpin structure that sequesters the cap, inhibits interactions with eukaryotic translation initiation factor 4E, and resists decapping. The 2G and 3G transcripts adopt an alternate structure with an elongated central helix, exposed splice donor residues, and an accessible cap. Extensive remodeling, achieved at the energetic cost of a G-C base pair, explains how a single 5'-guanosine modifies the function of a ~9-kilobase HIV-1 transcript.

PubMed: 32327595
DOI: 10.1126/science.aaz7959

実験手法

SOLUTION NMR