6VVE

Legionella pneumophila Lpg2603 kinase bound to IP6, Mn2+, and ADP

6VVE の概要

• エントリーDOI: 10.2210/pdb6vve/pdb
• 分子名称: Dot/Icm T4SS effector, INOSITOL HEXAKISPHOSPHATE, ADENOSINE-5'-DIPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: phosphorylation, kinase, ip6, adp, transferase
• 由来する生物種: Legionella pneumophila
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37651.08

構造登録者

Tomchick, D.R.,Tagliabracci, V.S.,Park, B.C. (登録日: 2020-02-17, 公開日: 2020-04-08, 最終更新日: 2024-11-06)

主引用文献

Sreelatha, A.,Nolan, C.,Park, B.C.,Pawlowski, K.,Tomchick, D.R.,Tagliabracci, V.S.
ALegionellaeffector kinase is activated by host inositol hexakisphosphate.
J.Biol.Chem., 295:6214-6224, 2020

PubMed Abstract: The transfer of a phosphate from ATP to a protein substrate, a modification known as protein phosphorylation, is catalyzed by protein kinases. Protein kinases play a crucial role in virtually every cellular activity. Recent studies of atypical protein kinases have highlighted the structural similarity of the kinase superfamily despite notable differences in primary amino acid sequence. Here, using a bioinformatics screen, we searched for putative protein kinases in the intracellular bacterial pathogen and identified the type 4 secretion system effector Lpg2603 as a remote member of the protein kinase superfamily. Employing an array of biochemical and structural biology approaches, including kinase assays and isothermal titration calorimetry, we show that Lpg2603 is an active protein kinase with several atypical structural features. Importantly, we found that the eukaryote-specific host signaling molecule inositol hexakisphosphate (IP6) is required for Lpg2603 kinase activity. Crystal structures of Lpg2603 in the apo-form and when bound to IP6 revealed an active-site rearrangement that allows for ATP binding and catalysis. Our results on the structure and activity of Lpg2603 reveal a unique mode of regulation of a protein kinase, provide the first example of a bacterial kinase that requires IP6 for its activation, and may aid future work on the function of this effector during pathogenesis.

PubMed: 32229585
DOI: 10.1074/jbc.RA120.013067

実験手法

X-RAY DIFFRACTION (1.85 Å)