6VSP

Structure of Serratia marcescens 2,3-butanediol dehydrogenase mutant Q247A

6VSP の概要

• エントリーDOI: 10.2210/pdb6vsp/pdb
• 分子名称: 2,3-butanediol dehydrogenase, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, GLYCEROL, ... (7 entities in total)
• 機能のキーワード: bdh, oxidoreductase
• 由来する生物種: Serratia marcescens
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 58092.02

構造登録者

Alahuhta, P.M.,Lunin, V.V. (登録日: 2020-02-11, 公開日: 2020-12-23, 最終更新日: 2023-10-11)

主引用文献

Subramanian, V.,Lunin, V.V.,Farmer, S.J.,Alahuhta, M.,Moore, K.T.,Ho, A.,Chaudhari, Y.B.,Zhang, M.,Himmel, M.E.,Decker, S.R.
Phylogenetics-based identification and characterization of a superior 2,3-butanediol dehydrogenase for Zymomonas mobilis expression.
Biotechnol Biofuels, 13:186-186, 2020

PubMed Abstract: Zymomonas mobilis has recently been shown to be capable of producing the valuable platform biochemical, 2,3-butanediol (2,3-BDO). Despite this capability, the production of high titers of 2,3-BDO is restricted by several physiological parameters. One such bottleneck involves the conversion of acetoin to 2,3-BDO, a step catalyzed by 2,3-butanediol dehydrogenase (Bdh). Several Bdh enzymes have been successfully expressed in Z. mobilis, although a highly active enzyme is yet to be identified for expression in this host. Here, we report the application of a phylogenetic approach to identify and characterize a superior Bdh, followed by validation of its structural attributes using a mutagenesis approach.

PubMed: 33292448
DOI: 10.1186/s13068-020-01820-x

実験手法

X-RAY DIFFRACTION (1.9 Å)