6VR8

Structure of a pseudomurein peptide ligase type E from Methanothermus fervidus

6VR8 の概要

• エントリーDOI: 10.2210/pdb6vr8/pdb
• 分子名称: Mur ligase middle domain protein, PHOSPHATE ION, MAGNESIUM ION, ... (7 entities in total)
• 機能のキーワード: pseudomurein, pseudomurein peptide ligase, archaeal cell wall, methanothermus fervidus, pmure, ligase
• 由来する生物種: Methanothermus fervidus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 55187.06

構造登録者

Carbone, V.,Schofield, L.R.,Sutherland-Smith, A.J.,Ronimus, R.S.,Subedi, B.P. (登録日: 2020-02-06, 公開日: 2021-08-11, 最終更新日: 2024-10-23)

主引用文献

Subedi, B.P.,Schofield, L.R.,Carbone, V.,Wolf, M.,Martin, W.F.,Ronimus, R.S.,Sutherland-Smith, A.J.
Structural characterisation of methanogen pseudomurein cell wall peptide ligases homologous to bacterial MurE/F murein peptide ligases.
Microbiology (Reading, Engl.), 168:-, 2022

PubMed Abstract: Archaea have diverse cell wall types, yet none are identical to bacterial peptidoglycan (murein). Methanogens and possess cell walls of pseudomurein, a structural analogue of murein. Pseudomurein differs from murein in containing the unique archaeal sugar -acetyltalosaminuronic acid instead of -acetylmuramic acid, β-1,3 glycosidic bonds in place of β-1,4 bonds and only l-amino acids in the peptide cross-links. We have determined crystal structures of methanogen pseudomurein peptide ligases (termed pMurE) from (Mfer762) and (Mth734) that are structurally most closely related to bacterial MurE peptide ligases. The homology of the archaeal pMurE and bacterial MurE enzymes is clear both in the overall structure and at the level of each of the three domains. In addition, we identified two UDP-binding sites in Mfer762 pMurE, one at the exterior surface of the interface of the N-terminal and middle domains, and a second site at an inner surface continuous with the highly conserved interface of the three domains. Residues involved in ATP binding in MurE are conserved in pMurE, suggesting that a similar ATP-binding pocket is present at the interface of the middle and the C-terminal domains of pMurE. The presence of pMurE ligases in members of the Methanobacteriales and Methanopyrales, that are structurally related to bacterial MurE ligases, supports the idea that the biosynthetic origins of archaeal pseudomurein and bacterial peptidoglycan cell walls are evolutionarily related.

PubMed: 36178458
DOI: 10.1099/mic.0.001235

実験手法

X-RAY DIFFRACTION (1.9 Å)