6VQ5

Crystal Structure of Epiphyas postvittana Pheromone Binding Protein 3

6VQ5 の概要

• エントリーDOI: 10.2210/pdb6vq5/pdb
• 分子名称: Pheromome Binding Protein, 2-(2-METHOXYETHOXY)ETHANOL (3 entities in total)
• 機能のキーワード: insect pheromone binding protein, insect odorant binding protein, transport protein
• 由来する生物種: Epiphyas postvittana (Light brown apple moth)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36851.95

構造登録者

Hamiaux, C.,Carraher, C. (登録日: 2020-02-04, 公開日: 2020-10-14, 最終更新日: 2024-10-23)

主引用文献

Hamiaux, C.,Carraher, C.,Lofstedt, C.,Corcoran, J.A.
Crystal structure of Epiphyas postvittana pheromone binding protein 3.
Sci Rep, 10:16366-16366, 2020

PubMed Abstract: The insect olfactory system operates as a well-choreographed ensemble of molecules which functions to selectively translate volatile chemical messages present in the environment into neuronal impulses that guide insect behaviour. Of these molecules, binding proteins are believed to transport hydrophobic odorant molecules across the aqueous lymph present in antennal sensilla to receptors present in olfactory sensory neurons. Though the exact mechanism through which these proteins operate is still under investigation, these carriers clearly play a critical role in determining what an insect can smell. Binding proteins that transport important sex pheromones are colloquially named pheromone binding proteins (PBPs). Here, we have produced a functional recombinant PBP from the horticultural pest, Epiphyas postvittana (EposPBP3), and experimentally solved its apo-structure through X-ray crystallography to a resolution of 2.60 Å. Structural comparisons with related lepidopteran PBPs further allowed us to propose models for the binding of pheromone components to EposPBP3. The data presented here represent the first structure of an olfactory-related protein from the tortricid family of moths, whose members cause billions of dollars in losses to agricultural producers each year. Knowledge of the structure of these important proteins will allow for subsequent studies in which novel, olfactory molecule-specific insecticides can be developed.

PubMed: 33004932
DOI: 10.1038/s41598-020-73294-8

実験手法

X-RAY DIFFRACTION (2.6 Å)