6VLW

Crystal Structure of 426cOD in Complex with VRC01 Fab

6VLW の概要

• エントリーDOI: 10.2210/pdb6vlw/pdb
• 分子名称: VRC01 Fab Heavy Chain, VRC01 Fab Light Chain, 426cOD, ... (10 entities in total)
• 機能のキーワード: hiv, 426c, outer domain, vrc01, 426cod, fab, cd4, antibody, immunogen, immune system, immune system-viral protein complex, immune system/viral protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 71511.99

構造登録者

Weidle, C.,Pancera, M. (登録日: 2020-01-27, 公開日: 2020-12-09, 最終更新日: 2024-10-16)

主引用文献

Lin, Y.R.,Parks, K.R.,Weidle, C.,Naidu, A.S.,Khechaduri, A.,Riker, A.O.,Takushi, B.,Chun, J.H.,Borst, A.J.,Veesler, D.,Stuart, A.,Agrawal, P.,Gray, M.,Pancera, M.,Huang, P.S.,Stamatatos, L.
HIV-1 VRC01 Germline-Targeting Immunogens Select Distinct Epitope-Specific B Cell Receptors.
Immunity, 53:840-851.e6, 2020

PubMed Abstract: Activating precursor B cell receptors of HIV-1 broadly neutralizing antibodies requires specifically designed immunogens. Here, we compared the abilities of three such germline-targeting immunogens against the VRC01-class receptors to activate the targeted B cells in transgenic mice expressing the germline VH of the VRC01 antibody but diverse mouse light chains. Immunogen-specific VRC01-like B cells were isolated at different time points after immunization, their VH and VL genes were sequenced, and the corresponding antibodies characterized. VRC01 B cell sub-populations with distinct cross-reactivity properties were activated by each immunogen, and these differences correlated with distinct biophysical and biochemical features of the germline-targeting immunogens. Our study indicates that the design of effective immunogens to activate B cell receptors leading to protective HIV-1 antibodies will require a better understanding of how the biophysical properties of the epitope and its surrounding surface on the germline-targeting immunogen influence its interaction with the available receptor variants in vivo.

PubMed: 33053332
DOI: 10.1016/j.immuni.2020.09.007

実験手法

X-RAY DIFFRACTION (3.42 Å)