6VBV

Structure of the bovine BBSome:ARL6:GTP complex

6VBV の概要

• エントリーDOI: 10.2210/pdb6vbv/pdb
• EMDBエントリー: 21145
• 分子名称: Bardet-Biedl syndrome 18 protein, CALCIUM ION, GUANOSINE-5'-TRIPHOSPHATE, ... (11 entities in total)
• 機能のキーワード: cilia, ciliopathy, complex, membrane-protein transport, protein transport
• 由来する生物種: Bos taurus (Bovine); 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 508169.76

構造登録者

Singh, S.K.,Gui, M.,Koh, F.,Yip, M.C.J.,Brown, A. (登録日: 2019-12-19, 公開日: 2020-01-29, 最終更新日: 2024-03-06)

主引用文献

Singh, S.K.,Gui, M.,Koh, F.,Yip, M.C.,Brown, A.
Structure and activation mechanism of the BBSome membrane protein trafficking complex.
Elife, 9:-, 2020

PubMed Abstract: Bardet-Biedl syndrome (BBS) is a currently incurable ciliopathy caused by the failure to correctly establish or maintain cilia-dependent signaling pathways. Eight proteins associated with BBS assemble into the BBSome, a key regulator of the ciliary membrane proteome. We report the electron cryomicroscopy (cryo-EM) structures of the native bovine BBSome in inactive and active states at 3.1 and 3.5 Å resolution, respectively. In the active state, the BBSome is bound to an Arf-family GTPase (ARL6/BBS3) that recruits the BBSome to ciliary membranes. ARL6 recognizes a composite binding site formed by BBS1 and BBS7 that is occluded in the inactive state. Activation requires an unexpected swiveling of the β-propeller domain of BBS1, the subunit most frequently implicated in substrate recognition, which widens a central cavity of the BBSome. Structural mapping of disease-causing mutations suggests that pathogenesis results from folding defects and the disruption of autoinhibition and activation.

PubMed: 31939736
DOI: 10.7554/eLife.53322

実験手法

ELECTRON MICROSCOPY (3.5 Å)