6V8O

RSC core

6V8O の概要

• エントリーDOI: 10.2210/pdb6v8o/pdb
• EMDBエントリー: 21098 21102 21103 21104 21105 21106 21107 21110 21114
• 分子名称: High temperature lethal protein 1, Chromatin structure-remodeling complex protein RSC58, Chromatin structure-remodeling complex subunit SFH1, ... (19 entities in total)
• 機能のキーワード: chromatin remodeler, rsc, gene regulation
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 22
• 化学式量合計: 1105461.98

構造登録者

Patel, A.B.,Moore, C.M.,Greber, B.J.,Nogales, E. (登録日: 2019-12-11, 公開日: 2020-01-15, 最終更新日: 2025-06-04)

主引用文献

Patel, A.B.,Moore, C.M.,Greber, B.J.,Luo, J.,Zukin, S.A.,Ranish, J.,Nogales, E.
Architecture of the chromatin remodeler RSC and insights into its nucleosome engagement.
Elife, 8:-, 2019

PubMed Abstract: Eukaryotic DNA is packaged into nucleosome arrays, which are repositioned by chromatin remodeling complexes to control DNA accessibility. The RSC (emodeling the tructure of hromatin) complex, a member of the SWI/SNF chromatin remodeler family, plays critical roles in genome maintenance, transcription, and DNA repair. Here, we report cryo-electron microscopy (cryo-EM) and crosslinking mass spectrometry (CLMS) studies of yeast RSC complex and show that RSC is composed of a rigid tripartite core and two flexible lobes. The core structure is scaffolded by an asymmetric Rsc8 dimer and built with the evolutionarily conserved subunits Sfh1, Rsc6, Rsc9 and Sth1. The flexible ATPase lobe, composed of helicase subunit Sth1, Arp7, Arp9 and Rtt102, is anchored to this core by the N-terminus of Sth1. Our cryo-EM analysis of RSC bound to a nucleosome core particle shows that in addition to the expected nucleosome-Sth1 interactions, RSC engages histones and nucleosomal DNA through one arm of the core structure, composed of the Rsc8 SWIRM domains, Sfh1 and Npl6. Our findings provide structural insights into the conserved assembly process for all members of the SWI/SNF family of remodelers, and illustrate how RSC selects, engages, and remodels nucleosomes.

PubMed: 31886770
DOI: 10.7554/eLife.54449

実験手法

ELECTRON MICROSCOPY (3.07 Å)