6V7U

Structure of a phage-encoded quorum sensing anti-activator, Aqs1

6V7U の概要

• エントリーDOI: 10.2210/pdb6v7u/pdb
• 分子名称: Quorum sensing anti-activator protein Aqs1 (2 entities in total)
• 機能のキーワード: prophage protein, viral protein
• 由来する生物種: Pseudomonas virus DMS3
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 15066.56

構造登録者

Shah, M.,Moraes, T.F.,Maxwell, K.L. (登録日: 2019-12-09, 公開日: 2020-12-16, 最終更新日: 2024-03-06)

主引用文献

Shah, M.,Taylor, V.L.,Bona, D.,Tsao, Y.,Stanley, S.Y.,Pimentel-Elardo, S.M.,McCallum, M.,Bondy-Denomy, J.,Howell, P.L.,Nodwell, J.R.,Davidson, A.R.,Moraes, T.F.,Maxwell, K.L.
A phage-encoded anti-activator inhibits quorum sensing in Pseudomonas aeruginosa.
Mol.Cell, 81:571-583.e6, 2021

PubMed Abstract: The arms race between bacteria and phages has led to the evolution of diverse anti-phage defenses, several of which are controlled by quorum-sensing pathways. In this work, we characterize a quorum-sensing anti-activator protein, Aqs1, found in Pseudomonas phage DMS3. We show that Aqs1 inhibits LasR, the master regulator of quorum sensing, and present the crystal structure of the Aqs1-LasR complex. The 69-residue Aqs1 protein also inhibits PilB, the type IV pilus assembly ATPase protein, which blocks superinfection by phages that require the pilus for infection. This study highlights the remarkable ability of small phage proteins to bind multiple host proteins and disrupt key biological pathways. As quorum sensing influences various anti-phage defenses, Aqs1 provides a mechanism by which infecting phages might simultaneously dampen multiple defenses. Because quorum-sensing systems are broadly distributed across bacteria, this mechanism of phage counter-defense may play an important role in phage-host evolutionary dynamics.

PubMed: 33412111
DOI: 10.1016/j.molcel.2020.12.011

実験手法

X-RAY DIFFRACTION (2.08 Å)