6V5C

Human Drosha and DGCR8 in complex with Primary MicroRNA (MP/RNA complex) - partially docked state

6V5C の概要

• エントリーDOI: 10.2210/pdb6v5c/pdb
• EMDBエントリー: 21051 21052
• 分子名称: Ribonuclease 3, Microprocessor complex subunit DGCR8, Pri-miR-16-2 (66-MER) (3 entities in total)
• 機能のキーワード: microprocessor, drosha, dgcr8, primary microrna, rna binding protein, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 273125.88

構造登録者

Partin, A.,Zhang, K.,Jeong, B.,Herrell, E.,Li, S.,Chiu, W.,Nam, Y. (登録日: 2019-12-04, 公開日: 2020-04-08, 最終更新日: 2024-03-06)

主引用文献

Partin, A.C.,Zhang, K.,Jeong, B.C.,Herrell, E.,Li, S.,Chiu, W.,Nam, Y.
Cryo-EM Structures of Human Drosha and DGCR8 in Complex with Primary MicroRNA.
Mol.Cell, 78:411-, 2020

PubMed Abstract: Metazoan microRNAs require specific maturation steps initiated by Microprocessor, comprising Drosha and DGCR8. Lack of structural information for the assembled complex has hindered an understanding of how Microprocessor recognizes primary microRNA transcripts (pri-miRNAs). Here we present a cryoelectron microscopy structure of human Microprocessor with a pri-miRNA docked in the active site, poised for cleavage. The basal junction is recognized by a four-way intramolecular junction in Drosha, triggered by the Belt and Wedge regions that clamp over the ssRNA. The belt is important for efficiency and accuracy of pri-miRNA processing. Two dsRBDs form a molecular ruler to measure the stem length between the two dsRNA-ssRNA junctions. The specific organization of the dsRBDs near the apical junction is independent of Drosha core domains, as observed in a second structure in the partially docked state. Collectively, we derive a molecular model to explain how Microprocessor recognizes a pri-miRNA and accurately identifies the cleavage site.

PubMed: 32220646
DOI: 10.1016/j.molcel.2020.02.016

実験手法

ELECTRON MICROSCOPY (4.4 Å)