6V1V

VIP3B (VIP3B_2160) adapted for crystallization

6V1V の概要

• エントリーDOI: 10.2210/pdb6v1v/pdb
• 分子名称: Vegetative insecticidal protein (1 entity in total)
• 機能のキーワード: insecticidal protein bacillus thuringiensis pore forming, toxin
• 由来する生物種: Bacillus thuringiensis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 360286.22

構造登録者

Evdokimov, A.G.,Zheng, M.,Moshiri, F.,Haas, J.,Lowder, C. (登録日: 2019-11-21, 公開日: 2019-12-18, 最終更新日: 2024-04-03)

主引用文献

Zheng, M.,Evdokimov, A.G.,Moshiri, F.,Lowder, C.,Haas, J.
Crystal structure of a Vip3B family insecticidal protein reveals a new fold and a unique tetrameric assembly.
Protein Sci., 29:824-829, 2020

PubMed Abstract: Vegetatively expressed insecticidal proteins (VIPs) produced by Bacillus thuringiensis fall into several classes of which the third, VIP3, is known for their activity against several key Lepidopteran pests of commercial broad acre crops and because their mode of action does not overlap with that of crystalline insecticidal proteins. The details of the VIP3 structure and mode of action have remained obscure for the quarter century that has passed since their discovery. In the present article, we report the first crystal structure of a full-length VIP3 protein. Crystallization of this target required multiple rounds of construct optimization and screening-over 200 individual sequences were expressed and tested. This protein adopts a novel global fold that combines domains with hitherto unreported topology and containing elements seemingly borrowed from carbohydrate-binding domains, lectins, or from other insecticidal proteins.

PubMed: 31840313
DOI: 10.1002/pro.3803

実験手法

X-RAY DIFFRACTION (3.189 Å)