6V0T

Crystal Structure of Catalytic Subunit of Bovine Pyruvate Dehydrogenase Phosphatase 1 - Catalytic Domain

「3N3C」から置き換えられました

6V0T の概要

• エントリーDOI: 10.2210/pdb6v0t/pdb
• 分子名称: [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial, MANGANESE (II) ION, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: pyruvate dehydrogenase phosphatase, pyruvate dehydrogenase, transferase, hydrolase
• 由来する生物種: Bos taurus (Bovine)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 53147.96

構造登録者

Guo, Y.,Qiu, W.,Ernst, S.R.,Carroll, D.W.,Hackert, M.L. (登録日: 2019-11-19, 公開日: 2019-12-18, 最終更新日: 2023-10-11)

主引用文献

Guo, Y.,Qiu, W.,Roche, T.E.,Hackert, M.L.
Crystal structure of the catalytic subunit of bovine pyruvate dehydrogenase phosphatase.
Acta Crystallogr.,Sect.F, 76:292-301, 2020

PubMed Abstract: Mammalian pyruvate dehydrogenase (PDH) activity is tightly regulated by phosphorylation and dephosphorylation, which is catalyzed by PDH kinase isomers and PDH phosphatase isomers, respectively. PDH phosphatase isomer 1 (PDP1) is a heterodimer consisting of a catalytic subunit (PDP1c) and a regulatory subunit (PDP1r). Here, the crystal structure of bovine PDP1c determined at 2.1 Å resolution is reported. The crystals belonged to space group P321, with unit-cell parameters a = b = 75.3, c = 173.2 Å. The structure was solved by molecular-replacement methods and refined to a final R factor of 21.9% (R = 24.7%). The final model consists of 402 of a possible 467 amino-acid residues of the PDP1c monomer, two Mn ions in the active site, an additional Mn ion coordinated by His410 and His414, two MnSO ion pairs at special positions near the crystallographic twofold symmetry axis and 226 water molecules. Several new features of the PDP1c structure are revealed. The requirements are described and plausible bases are deduced for the interaction of PDP1c with PDP1r and other components of the pyruvate dehydrogenase complex.

PubMed: 32627744
DOI: 10.1107/S2053230X20007943

実験手法

X-RAY DIFFRACTION (2.1 Å)