6V0K

Crystal structure of Penicillium verruculosum copalyl diphosphate synthase (PvCPS) alpha prenyltransferase domain

Summary for 6V0K

• Entry DOI: 10.2210/pdb6v0k/pdb
• Descriptor: Terpene synthase, GLYCEROL (3 entities in total)
• Functional Keywords: isoprenoid synthase, prenyltransferase, transferase
• Biological source: Talaromyces verruculosus
• Total number of polymer chains: 2
• Total formula weight: 69760.03

Authors

Christianson, D.W.,Ronnebaum, T.A. (deposition date: 2019-11-18, release date: 2020-01-29, Last modification date: 2023-10-11)

Primary citation

Ronnebaum, T.A.,Gupta, K.,Christianson, D.W.
Higher-order oligomerization of a chimeric alpha beta gamma bifunctional diterpene synthase with prenyltransferase and class II cyclase activities is concentration-dependent.
J.Struct.Biol., 210:107463-107463, 2020

PubMed Abstract: The unusual diterpene (C) synthase copalyl diphosphate synthase from Penicillium verruculosum (PvCPS) is the first bifunctional terpene synthase identified with both prenyltransferase and class II cyclase activities in a single polypeptide chain with αβγ domain architecture. The C-terminal prenyltransferase α domain generates geranylgeranyl diphosphate which is then cyclized to form copalyl diphosphate at the N-terminal βγ domain interface. We now demonstrate that PvCPS exists as a hexamer at high concentrations - a unique quaternary structure for known αβγ terpene synthases. Hexamer assembly is corroborated by a 2.41 Å-resolution crystal structure of the α domain prenyltransferase obtained from limited proteolysis of full-length PvCPS, as well as the ab initio model of full-length PvCPS derived from small-angle X-ray scattering data. Hexamerization of the prenyltransferase α domain appears to drive the hexamerization of full-length PvCPS. The PvCPS hexamer dissociates into lower-order species at lower concentrations, as evidenced by size-exclusion chromatography in-line with multiangle light scattering, sedimentation velocity analytical ultracentrifugation, and native polyacrylamide gel electrophoresis experiments, suggesting that oligomerization is concentration dependent. Even so, PvCPS oligomer assembly does not affect prenyltransferase activity in vitro.

PubMed: 31978464
DOI: 10.1016/j.jsb.2020.107463

Experimental method

X-RAY DIFFRACTION (2.41 Å)