6UWJ
Intermediate engineered variant of I-OnuI meganuclease with improved thermostability and partially altered specificity
Summary for 6UWJ
Entry DOI | 10.2210/pdb6uwj/pdb |
Descriptor | I-OnuI-e-Therm-hChr11v2, DNA (27-MER), CALCIUM ION, ... (5 entities in total) |
Functional Keywords | meganuclease, homing endonuclease, dna binding protein, dna binding protein-dna complex, dna binding protein/dna |
Biological source | synthetic construct More |
Total number of polymer chains | 3 |
Total formula weight | 50898.78 |
Authors | Werther, R.,Stoddard, B.L. (deposition date: 2019-11-05, release date: 2019-12-18, Last modification date: 2023-10-11) |
Primary citation | Lambert, A.R.,Hallinan, J.P.,Werther, R.,Glow, D.,Stoddard, B.L. Optimization of Protein Thermostability and Exploitation of Recognition Behavior to Engineer Altered Protein-DNA Recognition. Structure, 28:760-775.e8, 2020 Cited by PubMed: 32359399DOI: 10.1016/j.str.2020.04.009 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.852 Å) |
Structure validation
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