6UIX

Cryo-EM structure of human CALHM2 gap junction

6UIX の概要

• エントリーDOI: 10.2210/pdb6uix/pdb
• EMDBエントリー: 20788 20789 20790
• 分子名称: Calcium homeostasis modulator protein 2 (1 entity in total)
• 機能のキーワード: calcium homeostasis modulator, calhm2, gap junction, transport protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 22
• 化学式量合計: 818370.87

構造登録者

Lu, W.,Du, J.,Choi, W. (登録日: 2019-10-01, 公開日: 2019-11-27, 最終更新日: 2024-11-20)

主引用文献

Choi, W.,Clemente, N.,Sun, W.,Du, J.,Lu, W.
The structures and gating mechanism of human calcium homeostasis modulator 2.
Nature, 576:163-167, 2019

PubMed Abstract: Calcium homeostasis modulators (CALHMs) are voltage-gated, Ca-inhibited nonselective ion channels that act as major ATP release channels, and have important roles in gustatory signalling and neuronal toxicity. Dysfunction of CALHMs has previously been linked to neurological disorders. Here we present cryo-electron microscopy structures of the human CALHM2 channel in the Ca-free active or open state and in the ruthenium red (RUR)-bound inhibited state, at resolutions up to 2.7 Å. Our work shows that purified CALHM2 channels form both gap junctions and undecameric hemichannels. The protomer shows a mirrored arrangement of the transmembrane domains (helices S1-S4) relative to other channels with a similar topology, such as connexins, innexins and volume-regulated anion channels. Upon binding to RUR, we observed a contracted pore with notable conformational changes of the pore-lining helix S1, which swings nearly 60° towards the pore axis from a vertical to a lifted position. We propose a two-section gating mechanism in which the S1 helix coarsely adjusts, and the N-terminal helix fine-tunes, the pore size. We identified a RUR-binding site near helix S1 that may stabilize this helix in the lifted conformation, giving rise to channel inhibition. Our work elaborates on the principles of CALHM2 channel architecture and symmetry, and the mechanism that underlies channel inhibition.

PubMed: 31776515
DOI: 10.1038/s41586-019-1781-3

実験手法

ELECTRON MICROSCOPY (3.5 Å)