6UIG

Crystal structure of human monoclonal antibody H7.200 in complex with H7N9 hemagglutinin HA1

Summary for 6UIG

Entry DOI	10.2210/pdb6uig/pdb
Descriptor	Hemagglutinin, H7.200 Fab heavy chain, H7.200 Fab light chain, ... (4 entities in total)
Functional Keywords	influenza a, monoclonal antibody, antiviral protein
Biological source	Influenza A virus More
Total number of polymer chains	6
Total formula weight	163216.44
Authors	Dong, J.,Crowe, J.E. (deposition date: 2019-09-30, release date: 2020-10-07, Last modification date: 2024-10-30)
Primary citation	Dong, J.,Gilchuk, I.,Li, S.,Irving, R.,Goff, M.T.,Turner, H.L.,Ward, A.B.,Carnahan, R.H.,Crowe Jr., J.E. Anti-influenza H7 human antibody targets antigenic site in hemagglutinin head domain interface. J.Clin.Invest., 130:4734-4739, 2020 Cited by PubMed Abstract: Although broadly protective, stem-targeted Abs against the influenza A virus hemagglutinin (HA) have been well studied, very limited information is available on Abs that broadly recognize the head domain. We determined the crystal structure of the HA protein of the avian H7N9 influenza virus in complex with a pan-H7, non-neutralizing, protective human Ab. The structure revealed a B cell epitope in the HA head domain trimer interface (TI). This discovery of a second major protective TI epitope supports a model in which uncleaved HA trimers exist on the surface of infected cells in a highly dynamic state that exposes hidden HA head domain features. PubMed: 32749241 DOI: 10.1172/JCI136032 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (3.2 Å)

Structure validation