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6UHN

Crystal Structure of C148 mGFP-cDNA-1

Summary for 6UHN
Entry DOI10.2210/pdb6uhn/pdb
DescriptorC148 mGFP-cDNA-1, UNKNOWN LIGAND (3 entities in total)
Functional Keywordsgreen fluorescent protein, beta-barrel, dna, protein-dna conjugate, fluorescent protein
Biological sourceAequorea victoria
Total number of polymer chains2
Total formula weight62141.95
Authors
Winegar, P.W.,Hayes, O.G.,McMillan, J.R.,Figg, C.A.,Focia, P.J.,Mirkin, C.A. (deposition date: 2019-09-27, release date: 2020-03-18, Last modification date: 2023-11-15)
Primary citationWinegar, P.H.,Hayes, O.G.,McMillan, J.R.,Figg, C.A.,Focia, P.J.,Mirkin, C.A.
DNA-Directed Protein Packing within Single Crystals.
Chem, 6:1007-1017, 2020
Cited by
PubMed Abstract: Designed DNA-DNA interactions are investigated for their ability to modulate protein packing within single crystals of mutant green fluorescent proteins (mGFPs) functionalized with a single DNA strand (mGFP-DNA). We probe the effects of DNA sequence, length, and protein-attachment position on the formation and protein packing of mGFP-DNA crystals. Notably, when complementary mGFP-DNA conjugates are introduced to one another, crystals form with nearly identical packing parameters, regardless of sequence if the number of bases is equivalent. DNA complementarity is essential, because experiments with non-complementary sequences produce crystals with different protein arrangements. Importantly, the DNA length and its position of attachment on the protein markedly influence the formation of and protein packing within single crystals. This work shows how designed DNA interactions can be used to influence the growth and packing in X-ray diffraction quality protein single crystals and is thus an important step forward in protein crystal engineering.
PubMed: 33709040
DOI: 10.1016/j.chempr.2020.03.002
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.92 Å)
Structure validation

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数据于2024-10-30公开中

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