6UFH

Co-crystal structure of M. tuberculosis ileS T-box in complex with tRNA-3'-2'3'cyclic phosphate

Summary for 6UFH

• Entry DOI: 10.2210/pdb6ufh/pdb
• Descriptor: RNA (77-MER), RNA (167-MER), MAGNESIUM ION (3 entities in total)
• Functional Keywords: complex, riboregulator, riboswitch, rna
• Biological source: Mycobacterium tuberculosis; More
• Total number of polymer chains: 2
• Total formula weight: 79511.91

Authors

Battaglia, R.A.,Grigg, J.A.,Ke, A. (deposition date: 2019-09-24, release date: 2019-11-20, Last modification date: 2024-03-13)

Primary citation

Battaglia, R.A.,Grigg, J.C.,Ke, A.
Structural basis for tRNA decoding and aminoacylation sensing by T-box riboregulators.
Nat.Struct.Mol.Biol., 26:1106-1113, 2019

PubMed Abstract: T-box riboregulators are a class of cis-regulatory RNAs that govern the bacterial response to amino acid starvation by binding, decoding and reading the aminoacylation status of specific transfer RNAs. Here we provide a high-resolution crystal structure of a full-length T-box from Mycobacterium tuberculosis that explains tRNA decoding and aminoacylation sensing by this riboregulator. Overall, the T-box consists of decoding and aminoacylation sensing modules bridged by a rigid pseudoknot structure formed by the mid-region domains. Stem-I and the Stem-II S-turn assemble a claw-like decoding module, while the antiterminator, Stem-III, and the adjacent linker form a tightly interwoven aminoacylation sensing module. The uncharged tRNA is selectively recognized by an unexpected set of favorable contacts from the linker region in the aminoacylation sensing module. A complex structure with a charged tRNA mimic shows that the extra moiety dislodges the linker, which is indicative of the possible chain of events that lead to alternative base-pairing and altered expression output.

PubMed: 31740853
DOI: 10.1038/s41594-019-0327-6

Experimental method

X-RAY DIFFRACTION (3.104 Å)