6U7V

xRRM structure of spPof8

6U7V の概要

• エントリーDOI: 10.2210/pdb6u7v/pdb
• 分子名称: Protein pof8, NITRATE ION (3 entities in total)
• 機能のキーワード: telomere, telomerase, rna binding, gene regulation, ter1, xrrm
• 由来する生物種: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15551.24

構造登録者

Kim, J.-K.,Hu, X.,Yu, C.,Jun, H.-I.,Liu, J.,Sankaran, B.,Huang, L.,Qiao, F. (登録日: 2019-09-03, 公開日: 2020-09-09, 最終更新日: 2024-10-30)

主引用文献

Hu, X.,Kim, J.K.,Yu, C.,Jun, H.I.,Liu, J.,Sankaran, B.,Huang, L.,Qiao, F.
Quality-Control Mechanism for Telomerase RNA Folding in the Cell.
Cell Rep, 33:108568-108568, 2020

PubMed Abstract: Long non-coding RNAs can often fold into different conformations. Telomerase RNA, an essential component of the telomerase ribonucleoprotein (RNP) enzyme, must fold into a defined structure to fulfill its function with the protein catalytic subunit (TERT) and other accessory factors. However, the mechanism by which the correct folding of telomerase RNA is warranted in a cell is still unknown. Here we show that La-related protein Pof8 specifically recognizes the conserved pseudoknot region of telomerase RNA and instructs the binding of the Lsm2-8 complex to its mature 3' end, thus selectively protecting the correctly folded RNA from exonucleolytic degradation. In the absence of Pof8, TERT assembles with misfolded RNA and produces little telomerase activity. Therefore, Pof8 plays a key role in telomerase RNA folding quality control, ensuring that TERT only assembles with functional telomerase RNA to form active telomerase. Our finding reveals a mechanism for non-coding RNA folding quality control.

PubMed: 33378677
DOI: 10.1016/j.celrep.2020.108568

実験手法

X-RAY DIFFRACTION (1.42 Å)