6U4B

WbbM bifunctional glycosytransferase apo structure

6U4B の概要

• エントリーDOI: 10.2210/pdb6u4b/pdb
• 分子名称: WbbM protein, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: glycsoyltransferase, bifunctional, trimeric, wbbm, duf4422, transferase
• 由来する生物種: Klebsiella pneumoniae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 69746.27

構造登録者

Kimber, M.S.,Mallette, E.,Kamski-Hennekam, E.R.,Gitalis, R. (登録日: 2019-08-25, 公開日: 2020-01-22, 最終更新日: 2024-03-13)

主引用文献

Clarke, B.R.,Ovchinnikova, O.G.,Sweeney, R.P.,Kamski-Hennekam, E.R.,Gitalis, R.,Mallette, E.,Kelly, S.D.,Lowary, T.L.,Kimber, M.S.,Whitfield, C.
A bifunctional O-antigen polymerase structure reveals a new glycosyltransferase family.
Nat.Chem.Biol., 16:450-457, 2020

PubMed Abstract: Lipopolysaccharide O-antigen is an attractive candidate for immunotherapeutic strategies targeting antibiotic-resistant Klebsiella pneumoniae. Several K. pneumoniae O-serotypes are based on a shared O2a-antigen backbone repeating unit: (→ 3)-α-Galp-(1 → 3)-β-Galf-(1 →). O2a antigen is synthesized on undecaprenol diphosphate in a pathway involving the O2a polymerase, WbbM, before its export by an ATP-binding cassette transporter. This dual domain polymerase possesses a C-terminal galactopyranosyltransferase resembling known GT8 family enzymes, and an N-terminal DUF4422 domain identified here as a galactofuranosyltransferase defining a previously unrecognized family (GT111). Functional assignment of DUF4422 explains how galactofuranose is incorporated into various polysaccharides of importance in vaccine production and the food industry. In the 2.1-Å resolution structure, three WbbM protomers associate to form a flattened triangular prism connected to a central stalk that orients the active sites toward the membrane. The biochemical, structural and topological properties of WbbM offer broader insight into the mechanisms of assembly of bacterial cell-surface glycans.

PubMed: 32152541
DOI: 10.1038/s41589-020-0494-0

実験手法

X-RAY DIFFRACTION (2.1 Å)