6U42

Natively decorated ciliary doublet microtubule

これはPDB形式変換不可エントリーです。

6U42 の概要

• エントリーDOI: 10.2210/pdb6u42/pdb
• EMDBエントリー: 20631 20632 20633 20634 20635 20636 20637
• 分子名称: Tubulin beta, FAP45, RIB30, ... (41 entities in total)
• 機能のキーワード: motile cilia, microtubule doublet, repetitive structure, protein fibril
• 由来する生物種: Chlamydomonas reinhardtii; 詳細
• タンパク質・核酸の鎖数: 451
• 化学式量合計: 21385503.05

構造登録者

Ma, M.,Stoyanova, M.,Rademacher, G.,Dutcher, S.K.,Brown, A.,Zhang, R. (登録日: 2019-08-22, 公開日: 2019-11-13, 最終更新日: 2024-10-23)

主引用文献

Ma, M.,Stoyanova, M.,Rademacher, G.,Dutcher, S.K.,Brown, A.,Zhang, R.
Structure of the Decorated Ciliary Doublet Microtubule.
Cell, 179:909-922.e12, 2019

PubMed Abstract: The axoneme of motile cilia is the largest macromolecular machine of eukaryotic cells. In humans, impaired axoneme function causes a range of ciliopathies. Axoneme assembly, structure, and motility require a radially arranged set of doublet microtubules, each decorated in repeating patterns with non-tubulin components. We use single-particle cryo-electron microscopy to visualize and build an atomic model of the repeating structure of a native axonemal doublet microtubule, which reveals the identities, positions, repeat lengths, and interactions of 38 associated proteins, including 33 microtubule inner proteins (MIPs). The structure demonstrates how these proteins establish the unique architecture of doublet microtubules, maintain coherent periodicities along the axoneme, and stabilize the microtubules against the repeated mechanical stress induced by ciliary motility. Our work elucidates the architectural principles that underpin the assembly of this large, repetitive eukaryotic structure and provides a molecular basis for understanding the etiology of human ciliopathies.

PubMed: 31668805
DOI: 10.1016/j.cell.2019.09.030

実験手法

ELECTRON MICROSCOPY (3.4 Å)