6U0N

Asymmetrically open conformational state (Class II) of HIV-1 Env trimer BG505 SOSIP.664 in complex with sCD4 and E51 Fab

6U0N の概要

• エントリーDOI: 10.2210/pdb6u0n/pdb
• 関連するPDBエントリー: 6U0L
• EMDBエントリー: 20605 20608
• 分子名称: Envelope glycoprotein gp120, T-cell surface glycoprotein CD4, E51 Fab heavy chain, ... (8 entities in total)
• 機能のキーワード: bg505 sosip.664, e51, scd4, env, env open conformation, asymmetrically open env, viral protein
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• タンパク質・核酸の鎖数: 15
• 化学式量合計: 428071.67

構造登録者

Yang, Z.,Bjorkman, P.J. (登録日: 2019-08-14, 公開日: 2019-12-11, 最終更新日: 2024-10-23)

主引用文献

Yang, Z.,Wang, H.,Liu, A.Z.,Gristick, H.B.,Bjorkman, P.J.
Asymmetric opening of HIV-1 Env bound to CD4 and a coreceptor-mimicking antibody.
Nat.Struct.Mol.Biol., 26:1167-1175, 2019

PubMed Abstract: The human immunodeficiency virus (HIV-1) envelope (Env) glycoprotein, a (gp120-gp41) trimer, mediates fusion of viral and host cell membranes after gp120 binding to host receptor CD4. Receptor binding triggers conformational changes allowing coreceptor (CCR5) recognition through CCR5's tyrosine-sulfated amino (N) terminus, release of the gp41 fusion peptide and fusion. We present 3.3 Å and 3.5 Å cryo-EM structures of E51, a tyrosine-sulfated coreceptor-mimicking antibody, complexed with a CD4-bound open HIV-1 native-like Env trimer. Two classes of asymmetric Env interact with E51, revealing tyrosine-sulfated interactions with gp120 mimicking CCR5 interactions, and two conformations of gp120-gp41 protomers (A and B protomers in AAB and ABB trimers) that differ in their degree of CD4-induced trimer opening and induction of changes to the fusion peptide. By integrating the new structural information with previous closed and open envelope trimer structures, we modeled the order of conformational changes on the path to coreceptor binding site exposure and subsequent viral-host cell membrane fusion.

PubMed: 31792452
DOI: 10.1038/s41594-019-0344-5

実験手法

ELECTRON MICROSCOPY (3.5 Å)