6TZB

Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin in complex with 6'-SLNLN

6TZB の概要

• エントリーDOI: 10.2210/pdb6tzb/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900046
• 分子名称: Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (10 entities in total)
• 機能のキーワード: viral protein
• 由来する生物種: Influenza A virus (strain A/Hong Kong/1/1968 H3N2); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 174944.99

構造登録者

Wu, N.C.,Wilson, I.A. (登録日: 2019-08-12, 公開日: 2019-10-30, 最終更新日: 2024-11-13)

主引用文献

Wu, N.C.,Otwinowski, J.,Thompson, A.J.,Nycholat, C.M.,Nourmohammad, A.,Wilson, I.A.
Major antigenic site B of human influenza H3N2 viruses has an evolving local fitness landscape.
Nat Commun, 11:1233-1233, 2020

PubMed Abstract: Antigenic drift of influenza virus hemagglutinin (HA) is enabled by facile evolvability. However, HA antigenic site B, which has become immunodominant in recent human H3N2 influenza viruses, is also evolutionarily constrained by its involvement in receptor binding. Here, we employ deep mutational scanning to probe the local fitness landscape of HA antigenic site B in six different human H3N2 strains spanning from 1968 to 2016. We observe that the fitness landscape of HA antigenic site B can be very different between strains. Sequence variants that exhibit high fitness in one strain can be deleterious in another, indicating that the evolutionary constraints of antigenic site B have changed over time. Structural analysis suggests that the local fitness landscape of antigenic site B can be reshaped by natural mutations via modulation of the receptor-binding mode. Overall, these findings elucidate how influenza virus continues to explore new antigenic space despite strong functional constraints.

PubMed: 32144244
DOI: 10.1038/s41467-020-15102-5

実験手法

X-RAY DIFFRACTION (2.243 Å)