6TZ7

Crystal Structure of Aspergillus fumigatus Calcineurin A, Calcineurin B, FKBP12 and FK506 (Tacrolimus)

6TZ7 の概要

• エントリーDOI: 10.2210/pdb6tz7/pdb
• 分子名称: Serine/threonine-protein phosphatase 2B catalytic subunit, Calcineurin Ca2+-binding regulatory subunit CnaB, FK506-binding protein 1A, ... (10 entities in total)
• 機能のキーワード: calcineurin, fk506, hydrolase-isomerase-calcium binding complex, hydrolase/isomerase/calcium binding
• 由来する生物種: Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 79808.44

構造登録者

Fox III, D.,Horanyi, P.S. (登録日: 2019-08-10, 公開日: 2019-09-18, 最終更新日: 2023-10-11)

主引用文献

Juvvadi, P.R.,Fox 3rd, D.,Bobay, B.G.,Hoy, M.J.,Gobeil, S.M.C.,Venters, R.A.,Chang, Z.,Lin, J.J.,Averette, A.F.,Cole, D.C.,Barrington, B.C.,Wheaton, J.D.,Ciofani, M.,Trzoss, M.,Li, X.,Lee, S.C.,Chen, Y.L.,Mutz, M.,Spicer, L.D.,Schumacher, M.A.,Heitman, J.,Steinbach, W.J.
Harnessing calcineurin-FK506-FKBP12 crystal structures from invasive fungal pathogens to develop antifungal agents.
Nat Commun, 10:4275-4275, 2019

PubMed Abstract: Calcineurin is important for fungal virulence and a potential antifungal target, but compounds targeting calcineurin, such as FK506, are immunosuppressive. Here we report the crystal structures of calcineurin catalytic (CnA) and regulatory (CnB) subunits complexed with FK506 and the FK506-binding protein (FKBP12) from human fungal pathogens (Aspergillus fumigatus, Candida albicans, Cryptococcus neoformans and Coccidioides immitis). Fungal calcineurin complexes are similar to the mammalian complex, but comparison of fungal and human FKBP12 (hFKBP12) reveals conformational differences in the 40s and 80s loops. NMR analysis, molecular dynamic simulations, and mutations of the A. fumigatus CnA/CnB-FK506-FKBP12-complex identify a Phe88 residue, not conserved in hFKBP12, as critical for binding and inhibition of fungal calcineurin. These differences enable us to develop a less immunosuppressive FK506 analog, APX879, with an acetohydrazine substitution of the C22-carbonyl of FK506. APX879 exhibits reduced immunosuppressive activity and retains broad-spectrum antifungal activity and efficacy in a murine model of invasive fungal infection.

PubMed: 31537789
DOI: 10.1038/s41467-019-12199-1

実験手法

X-RAY DIFFRACTION (2.5 Å)