Summary for 6TUI
| Entry DOI | 10.2210/pdb6tui/pdb |
| Related | 6TOA 6TSU 6TSV |
| EMDB information | 10541 10542 10565 10566 10569 10571 10592 |
| Descriptor | Phage major capsid protein, HK97 family, Uncharacterized protein, Tail tube protein Rcc01691, ... (8 entities in total) |
| Functional Keywords | "gene transfer agent", "virion", "genome release", "hk97", virus |
| Biological source | Rhodobacter capsulatus SB 1003 More |
| Total number of polymer chains | 52 |
| Total formula weight | 1563657.67 |
| Authors | Bardy, P.,Fuzik, T.,Hrebik, D.,Pantucek, R.,Beatty, J.T.,Plevka, P. (deposition date: 2020-01-07, release date: 2020-10-14, Last modification date: 2024-05-22) |
| Primary citation | Bardy, P.,Fuzik, T.,Hrebik, D.,Pantucek, R.,Beatty, J.T.,Plevka, P. Virion of empty GTA particle Nat Commun, 11:3034-, 2020 Cited by PubMed Abstract: Alphaproteobacteria, which are the most abundant microorganisms of temperate oceans, produce phage-like particles called gene transfer agents (GTAs) that mediate lateral gene exchange. However, the mechanism by which GTAs deliver DNA into cells is unknown. Here we present the structure of the GTA of Rhodobacter capsulatus (RcGTA) and describe the conformational changes required for its DNA ejection. The structure of RcGTA resembles that of a tailed phage, but it has an oblate head shortened in the direction of the tail axis, which limits its packaging capacity to less than 4,500 base pairs of linear double-stranded DNA. The tail channel of RcGTA contains a trimer of proteins that possess features of both tape measure proteins of long-tailed phages from the family Siphoviridae and tail needle proteins of short-tailed phages from the family Podoviridae. The opening of a constriction within the RcGTA baseplate enables the ejection of DNA into bacterial periplasm. PubMed: 32541663DOI: 10.1038/s41467-020-16669-9 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (10.47 Å) |
Structure validation
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