6TSK

Beta-galactosidase in complex with L-ribose

6TSK の概要

• エントリーDOI: 10.2210/pdb6tsk/pdb
• EMDBエントリー: 10563 10564
• 分子名称: Beta-galactosidase, MAGNESIUM ION, beta-L-ribopyranose, ... (4 entities in total)
• 機能のキーワード: bgal, l-ribose, sugar binding protein
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 474279.08

構造登録者

Saur, M.,Hartshorn, M.J.,Dong, J.,Reeks, J.,Bunkoczi, G.,Jhoti, H.,Williams, P.A. (登録日: 2019-12-20, 公開日: 2020-01-08, 最終更新日: 2024-05-22)

主引用文献

Saur, M.,Hartshorn, M.J.,Dong, J.,Reeks, J.,Bunkoczi, G.,Jhoti, H.,Williams, P.A.
Fragment-based drug discovery using cryo-EM.
Drug Discov Today, 25:485-490, 2020

PubMed Abstract: Recent advances in electron cryo-microscopy (cryo-EM) structure determination have pushed the resolutions obtainable by the method into the range widely considered to be of utility for drug discovery. Here, we review the use of cryo-EM in fragment-based drug discovery (FBDD) based on in-house method development. We demonstrate not only that cryo-EM can reveal details of the molecular interactions between fragments and a protein, but also that the current reproducibility, quality, and throughput are compatible with FBDD. We exemplify this using the test system β-galactosidase (Bgal) and the oncology target pyruvate kinase 2 (PKM2).

PubMed: 31877353
DOI: 10.1016/j.drudis.2019.12.006

実験手法

ELECTRON MICROSCOPY (2.3 Å)