6TSF

Crystal structure of human L ferritin (HuLf) Fe(III)-loaded for 60 minutes

これはPDB形式変換不可エントリーです。

6TSF の概要

• エントリーDOI: 10.2210/pdb6tsf/pdb
• 関連するPDBエントリー: 6TR9 6TRZ 6TS0 6TS1 6TSA
• 分子名称: Ferritin light chain, FE (III) ION, CADMIUM ION, ... (5 entities in total)
• 機能のキーワード: human l ferritin, hulf, fe(iii), trinuclear fe(iii) cluster, metal binding protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20935.08

構造登録者

Pozzi, C.,Ciambellotti, S.,Turano, P.,Mangani, S. (登録日: 2019-12-20, 公開日: 2020-02-19, 最終更新日: 2024-01-24)

主引用文献

Ciambellotti, S.,Pozzi, C.,Mangani, S.,Turano, P.
Iron Biomineral Growth from the Initial Nucleation Seed in L-Ferritin.
Chemistry, 26:5770-5773, 2020

PubMed Abstract: X-ray structures of homopolymeric human L-ferritin and horse spleen ferritin were solved by freezing protein crystals at different time intervals after exposure to a ferric salt and revealed the growth of an octa-nuclear iron cluster on the inner surface of the protein cage with a key role played by some glutamate residues. An atomic resolution view of how the cluster formation develops starting from a (μ -oxo)tris[(μ -glutamato-κO:κO')](glutamato-κO)(diaquo)triiron(III) seed is provided. The results support the idea that iron biomineralization in ferritin is a process initiating at the level of the protein surface, capable of contributing coordination bonds and electrostatic guidance.

PubMed: 32027764
DOI: 10.1002/chem.202000064

実験手法

X-RAY DIFFRACTION (2.09 Å)