6TQO

rrn anti-termination complex

6TQO の概要

• エントリーDOI: 10.2210/pdb6tqo/pdb
• EMDBエントリー: 10548
• 分子名称: Inositol monophosphatase, DNA-directed RNA polymerase subunit beta', 30S ribosomal protein S4, ... (16 entities in total)
• 機能のキーワード: rrn, anti-termination complex, rnap, nus factors, suhb, s4, transcription
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 15
• 化学式量合計: 626003.20

構造登録者

Huang, Y.H.,Wahl, M.C.,Loll, B.,Hilal, T.,Said, N. (登録日: 2019-12-17, 公開日: 2020-08-05, 最終更新日: 2025-07-09)

主引用文献

Huang, Y.H.,Hilal, T.,Loll, B.,Burger, J.,Mielke, T.,Bottcher, C.,Said, N.,Wahl, M.C.
Structure-Based Mechanisms of a Molecular RNA Polymerase/Chaperone Machine Required for Ribosome Biosynthesis.
Mol.Cell, 79:1024-1036.e5, 2020

PubMed Abstract: Bacterial ribosomal RNAs are synthesized by a dedicated, conserved transcription-elongation complex that transcribes at high rates, shields RNA polymerase from premature termination, and supports co-transcriptional RNA folding, modification, processing, and ribosomal subunit assembly by presently unknown mechanisms. We have determined cryo-electron microscopy structures of complete Escherichia coli ribosomal RNA transcription elongation complexes, comprising RNA polymerase; DNA; RNA bearing an N-utilization-site-like anti-termination element; Nus factors A, B, E, and G; inositol mono-phosphatase SuhB; and ribosomal protein S4. Our structures and structure-informed functional analyses show that fast transcription and anti-termination involve suppression of NusA-stabilized pausing, enhancement of NusG-mediated anti-backtracking, sequestration of the NusG C-terminal domain from termination factor ρ, and the ρ blockade. Strikingly, the factors form a composite RNA chaperone around the RNA polymerase RNA-exit tunnel, which supports co-transcriptional RNA folding and annealing of distal RNA regions. Our work reveals a polymerase/chaperone machine required for biosynthesis of functional ribosomes.

PubMed: 32871103
DOI: 10.1016/j.molcel.2020.08.010

実験手法

ELECTRON MICROSCOPY (3.8 Å)