6TQJ

Crystal structure of the c14 ring of the F1FO ATP synthase from spinach chloroplast

Summary for 6TQJ

• Entry DOI: 10.2210/pdb6tqj/pdb
• Descriptor: ATP synthase subunit c, chloroplastic, EICOSANE, (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate, ... (4 entities in total)
• Functional Keywords: atp synthase, c ring, spinach, chloroplast, membrane protein
• Biological source: Spinacia oleracea (Spinach)
• Total number of polymer chains: 14
• Total formula weight: 119385.89

Authors

Kovalev, K.,Gushchin, I.,Vlasov, A.,Round, E.,Polovinkin, V.,Gordeliy, V. (deposition date: 2019-12-16, release date: 2019-12-25, Last modification date: 2024-01-24)

Primary citation

Vlasov, A.V.,Kovalev, K.V.,Marx, S.H.,Round, E.S.,Gushchin, I.Y.,Polovinkin, V.A.,Tsoy, N.M.,Okhrimenko, I.S.,Borshchevskiy, V.I.,Buldt, G.D.,Ryzhykau, Y.L.,Rogachev, A.V.,Chupin, V.V.,Kuklin, A.I.,Dencher, N.A.,Gordeliy, V.I.
Unusual features of the c-ring of F1FOATP synthases.
Sci Rep, 9:18547-18547, 2019

PubMed Abstract: Membrane integral ATP synthases produce adenosine triphosphate, the universal "energy currency" of most organisms. However, important details of proton driven energy conversion are still unknown. We present the first high-resolution structure (2.3 Å) of the in meso crystallized c-ring of 14 subunits from spinach chloroplasts. The structure reveals molecular mechanisms of intersubunit contacts in the c-ring, and it shows additional electron densities inside the c-ring which form circles parallel to the membrane plane. Similar densities were found in all known high-resolution structures of c-rings of FF ATP synthases from archaea and bacteria to eukaryotes. The densities might originate from isoprenoid quinones (such as coenzyme Q in mitochondria and plastoquinone in chloroplasts) that is consistent with differential UV-Vis spectroscopy of the c-ring samples, unusually large distance between polar/apolar interfaces inside the c-ring and universality among different species. Although additional experiments are required to verify this hypothesis, coenzyme Q and its analogues known as electron carriers of bioenergetic chains may be universal cofactors of ATP synthases, stabilizing c-ring and prevent ion leakage through it.

PubMed: 31811229
DOI: 10.1038/s41598-019-55092-z

Experimental method

X-RAY DIFFRACTION (2.3 Å)