6TO2

Crystal structure of CYP154C5 from Nocardia farcinica in complex with 5alpha-Androstan-3-one

6TO2 の概要

• エントリーDOI: 10.2210/pdb6to2/pdb
• 分子名称: Cytochrome P450 monooxygenase, PROTOPORPHYRIN IX CONTAINING FE, (5~{S},8~{S},9~{S},10~{S},13~{S},14~{S})-10,13-dimethyl-1,2,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydrocyclopenta[a]phenanthren-3-one, ... (5 entities in total)
• 機能のキーワード: cytochrome p450, steroid hydroxylating monooxygenase, steroid binding, oxidoreductase-substrate complex, oxidoreductase
• 由来する生物種: Nocardia farcinica IFM 10152
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 92291.98

構造登録者

Rodriguez, A.,Kluenemann, T.,Blankenfeldt, W.,Schallmey, A. (登録日: 2019-12-11, 公開日: 2020-11-18, 最終更新日: 2024-10-23)

主引用文献

Bracco, P.,Wijma, H.J.,Nicolai, B.,Buitrago, J.A.R.,Klunemann, T.,Vila, A.,Schrepfer, P.,Blankenfeldt, W.,Janssen, D.B.,Schallmey, A.
CYP154C5 Regioselectivity in Steroid Hydroxylation Explored by Substrate Modifications and Protein Engineering*.
Chembiochem, 22:1099-1110, 2021

PubMed Abstract: CYP154C5 from Nocardia farcinica is a P450 monooxygenase able to hydroxylate a range of steroids with high regio- and stereoselectivity at the 16α-position. Using protein engineering and substrate modifications based on the crystal structure of CYP154C5, an altered regioselectivity of the enzyme in steroid hydroxylation had been achieved. Thus, conversion of progesterone by mutant CYP154C5 F92A resulted in formation of the corresponding 21-hydroxylated product 11-deoxycorticosterone in addition to 16α-hydroxylation. Using MD simulation, this altered regioselectivity appeared to result from an alternative binding mode of the steroid in the active site of mutant F92A. MD simulation further suggested that the entrance of water to the active site caused higher uncoupling in this mutant. Moreover, exclusive 15α-hydroxylation was observed for wild-type CYP154C5 in the conversion of 5α-androstan-3-one, lacking an oxy-functional group at C17. Overall, our data give valuable insight into the structure-function relationship of this cytochrome P450 monooxygenase for steroid hydroxylation.

PubMed: 33145893
DOI: 10.1002/cbic.202000735

実験手法

X-RAY DIFFRACTION (2 Å)