6TNP

Crystal structure of the ScFv-5E5 in complex with a Tn glycopeptide

6TNP の概要

• エントリーDOI: 10.2210/pdb6tnp/pdb
• 分子名称: Light chain of our ScFv-5E5, Heavy chain of our ScFv-5E5, (2~{S})-~{N}-[2-[[(2~{S})-1-[[(2~{S},3~{R})-3-[(2~{S},3~{R},4~{R},5~{R},6~{R})-3-acetamido-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-2-yl]oxy-1-[[(2~{S})-1-[(2~{S})-2-aminocarbonylpyrrolidin-1-yl]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-butan-2-yl]amino]-3-oxidanyl-1-oxidanylidene-propan-2-yl]amino]-2-oxidanylidene-ethyl]-1-[(2~{S})-2-azanylpropanoyl]pyrrolidine-2-carboxamide, ... (4 entities in total)
• 機能のキーワード: antibody, scfv, 5e5, anti-tn antibody, muc1, cancer, immune system
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 156140.52

構造登録者

Macias-Leon, J.,Corzana, F.,Hurtado-Guerrero, R. (登録日: 2019-12-09, 公開日: 2020-11-18, 最終更新日: 2024-11-13)

主引用文献

Macias-Leon, J.,Bermejo, I.A.,Asin, A.,Garcia-Garcia, A.,Companon, I.,Jimenez-Moreno, E.,Coelho, H.,Mangini, V.,Albuquerque, I.S.,Marcelo, F.,Asensio, J.L.,Bernardes, G.J.L.,Joshi, H.J.,Fiammengo, R.,Blixt, O.,Hurtado-Guerrero, R.,Corzana, F.
Structural characterization of an unprecedented lectin-like antitumoral anti-MUC1 antibody.
Chem.Commun.(Camb.), 56:15137-15140, 2020

PubMed Abstract: The molecular basis of antibody 5E5, which recognizes the entire GalNAc unit as a primary epitope is disclosed. The antibody's contacts with the peptide are mostly limited to two residues, allowing it to show some degree of promiscuity. These findings open the door to the chemical design of peptide-mimetics for developing efficient anti-cancer vaccines and diagnostic tools.

PubMed: 33211039
DOI: 10.1039/d0cc06349e

実験手法

X-RAY DIFFRACTION (3 Å)