6TJV

Structure of the NDH-1MS complex from Thermosynechococcus elongatus

6TJV の概要

• エントリーDOI: 10.2210/pdb6tjv/pdb
• EMDBエントリー: 10513
• 分子名称: NAD(P)H-quinone oxidoreductase subunit 1, NAD(P)H-quinone oxidoreductase subunit J, NAD(P)H-quinone oxidoreductase subunit K, ... (26 entities in total)
• 機能のキーワード: carbon concentrating photosynthetic complex i, proton pump, membrane protein
• 由来する生物種: Thermosynechococcus elongatus (strain BP-1); 詳細
• タンパク質・核酸の鎖数: 18
• 化学式量合計: 511378.09

構造登録者

Schuller, J.M.,Saura, P.,Thiemann, J.,Schuller, S.K.,Gamiz-Hernandez, A.P.,Kurisu, G.,Nowaczyk, M.M.,Kaila, V.R.I. (登録日: 2019-11-27, 公開日: 2020-02-19, 最終更新日: 2025-10-01)

主引用文献

Schuller, J.M.,Saura, P.,Thiemann, J.,Schuller, S.K.,Gamiz-Hernandez, A.P.,Kurisu, G.,Nowaczyk, M.M.,Kaila, V.R.I.
Redox-coupled proton pumping drives carbon concentration in the photosynthetic complex I.
Nat Commun, 11:494-494, 2020

PubMed Abstract: Photosynthetic organisms capture light energy to drive their energy metabolism, and employ the chemical reducing power to convert carbon dioxide (CO) into organic molecules. Photorespiration, however, significantly reduces the photosynthetic yields. To survive under low CO concentrations, cyanobacteria evolved unique carbon-concentration mechanisms that enhance the efficiency of photosynthetic CO fixation, for which the molecular principles have remained unknown. We show here how modular adaptations enabled the cyanobacterial photosynthetic complex I to concentrate CO using a redox-driven proton-pumping machinery. Our cryo-electron microscopy structure at 3.2 Å resolution shows a catalytic carbonic anhydrase module that harbours a Zn active site, with connectivity to proton-pumping subunits that are activated by electron transfer from photosystem I. Our findings illustrate molecular principles in the photosynthetic complex I machinery that enabled cyanobacteria to survive in drastically changing CO conditions.

PubMed: 31980611
DOI: 10.1038/s41467-020-14347-4

実験手法

ELECTRON MICROSCOPY (3.2 Å)