6TIU

DROSOPHILA GTP-TUBULIN Y222F MUTANT

6TIU の概要

• エントリーDOI: 10.2210/pdb6tiu/pdb
• 関連するPDBエントリー: 6TIS
• 分子名称: Tubulin alpha-1 chain, Tubulin beta-1 chain, Stathmin-4, ... (7 entities in total)
• 機能のキーワード: microtubule, microtubule dynamics, microtule nucleation, cell cycle
• 由来する生物種: Drosophila melanogaster (Fruit fly); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 220299.40

構造登録者

Gigant, B. (登録日: 2019-11-22, 公開日: 2021-01-27, 最終更新日: 2024-01-24)

主引用文献

Ayukawa, R.,Iwata, S.,Imai, H.,Kamimura, S.,Hayashi, M.,Ngo, K.X.,Minoura, I.,Uchimura, S.,Makino, T.,Shirouzu, M.,Shigematsu, H.,Sekimoto, K.,Gigant, B.,Muto, E.
GTP-dependent formation of straight tubulin oligomers leads to microtubule nucleation.
J.Cell Biol., 220:-, 2021

PubMed Abstract: Nucleation of microtubules (MTs) is essential for cellular activities, but its mechanism is unknown because of the difficulty involved in capturing rare stochastic events in the early stage of polymerization. Here, combining rapid flush negative stain electron microscopy (EM) and kinetic analysis, we demonstrate that the formation of straight oligomers of critical size is essential for nucleation. Both GDP and GTP tubulin form single-stranded oligomers with a broad range of curvatures, but upon nucleation, the curvature distribution of GTP oligomers is shifted to produce a minor population of straight oligomers. With tubulin having the Y222F mutation in the β subunit, the proportion of straight oligomers increases and nucleation accelerates. Our results support a model in which GTP binding generates a minor population of straight oligomers compatible with lateral association and further growth to MTs. This study suggests that cellular factors involved in nucleation promote it via stabilization of straight oligomers.

PubMed: 33544140
DOI: 10.1083/jcb.202007033

実験手法

X-RAY DIFFRACTION (3.571 Å)