6TIG

Structure of the N terminal domain of Bc2L-C lectin (1-131) in complex with Globo H (H-type 3) antigen

これはPDB形式変換不可エントリーです。

6TIG の概要

• エントリーDOI: 10.2210/pdb6tig/pdb
• 分子名称: Lectin, alpha-L-fucopyranose-(1-2)-beta-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-galactopyranose-(1-3)-alpha-D-galactopyranose, alpha-L-fucopyranose-(1-2)-beta-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-galactopyranose-(1-3)-alpha-D-galactopyranose-(1-4)-beta-D-galactopyranose, ... (4 entities in total)
• 機能のキーワード: lectin, tnf-like, globo h, sugar binding protein
• 由来する生物種: Burkholderia cenocepacia J2315
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 44431.98

構造登録者

Varrot, A.,Bermeo, R. (登録日: 2019-11-22, 公開日: 2020-01-22, 最終更新日: 2024-01-24)

主引用文献

Bermeo, R.,Bernardi, A.,Varrot, A.
BC2L-C N-Terminal Lectin Domain Complexed with Histo Blood Group Oligosaccharides Provides New Structural Information.
Molecules, 25:-, 2020

PubMed Abstract: Lectins mediate adhesion of pathogens to host tissues, filling in a key role in the first steps of infection. Belonging to the opportunistic pathogen , BC2L-C is a superlectin with dual carbohydrate specificity, believed to mediate cross-linking between bacteria and host cells. Its C-terminal domain binds to bacterial mannosides while its N-terminal domain (BCL2-CN) recognizes fucosylated human epitopes. BC2L-CN presents a tumor necrosis factor alpha (TNF-) fold previously unseen in lectins with a novel fucose binding mode. We report, here, the production of a novel recombinant form of BC2L-CN (rBC2L-CN2), which allowed better protein stability and unprecedented co-crystallization with oligosaccharides. Isothermal calorimetry measurements showed no detrimental effect on ligand binding and data were obtained on the binding of Globo H hexasaccharide and l-galactose. Crystal structures of rBC2L-CN2 were solved in complex with two blood group antigens: H-type 1 and H-type 3 (Globo H) by X-ray crystallography. They provide new structural information on the binding site, of importance for the structural-based design of glycodrugs as new antimicrobials with antiadhesive properties.

PubMed: 31936166
DOI: 10.3390/molecules25020248

実験手法

X-RAY DIFFRACTION (1.9 Å)