6TER

Crystal structure of a galactokinase from Bifidobacterium infantis in complex with Galactose

6TER の概要

• エントリーDOI: 10.2210/pdb6ter/pdb
• 分子名称: Galactokinase, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, GLYCEROL, ... (11 entities in total)
• 機能のキーワード: gal, complex, galactokinase, transferase
• 由来する生物種: Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 190943.68

構造登録者

Keenan, T.,Parmeggiani, F.,Fontenelle, C.Q.,Malassis, J.,Vendeville, J.,Offen, W.A.,Both, P.,Huang, K.,Marchesi, A.,Heyam, A.,Young, C.,Charnock, S.,Davies, G.J.,Linclau, B.,Flitsch, S.L.,Fascione, M.A. (登録日: 2019-11-12, 公開日: 2020-06-10, 最終更新日: 2024-01-24)

主引用文献

Keenan, T.,Parmeggiani, F.,Malassis, J.,Fontenelle, C.Q.,Vendeville, J.B.,Offen, W.,Both, P.,Huang, K.,Marchesi, A.,Heyam, A.,Young, C.,Charnock, S.J.,Davies, G.J.,Linclau, B.,Flitsch, S.L.,Fascione, M.A.
Profiling Substrate Promiscuity of Wild-Type Sugar Kinases for Multi-fluorinated Monosaccharides.
Cell Chem Biol, 27:1199-, 2020

PubMed Abstract: Fluorinated sugar-1-phosphates are of emerging importance as intermediates in the chemical and biocatalytic synthesis of modified oligosaccharides, as well as probes for chemical biology. Here we present a systematic study of the activity of a wide range of anomeric sugar kinases (galacto- and N-acetylhexosamine kinases) against a panel of fluorinated monosaccharides, leading to the first examples of polyfluorinated substrates accepted by this class of enzymes. We have discovered four new N-acetylhexosamine kinases with a different substrate scope, thus expanding the number of homologs available in this subclass of kinases. Lastly, we have solved the crystal structure of a galactokinase in complex with 2-deoxy-2-fluorogalactose, giving insight into changes in the active site that may account for the specificity of the enzyme toward certain substrate analogs.

PubMed: 32619452
DOI: 10.1016/j.chembiol.2020.06.005

実験手法

X-RAY DIFFRACTION (1.68 Å)