6TDA

Structure of SWI/SNF chromatin remodeler RSC bound to a nucleosome

6TDA の概要

• エントリーDOI: 10.2210/pdb6tda/pdb
• EMDBエントリー: 10465
• 分子名称: Histone H3.2, Chromatin structure-remodeling complex subunit RSC9, Chromatin structure-remodeling complex protein RSC6, ... (20 entities in total)
• 機能のキーワード: chromatin remodeler dna binding nucleosome binding atpase transcription, dna binding protein
• 由来する生物種: Xenopus laevis (African clawed frog); 詳細
• タンパク質・核酸の鎖数: 23
• 化学式量合計: 1054129.14

構造登録者

Wagner, F.R.,Dienemann, C.,Wang, H.,Stuetzer, A.,Tegunov, D.,Urlaub, H.,Cramer, P. (登録日: 2019-11-08, 公開日: 2020-03-18, 最終更新日: 2024-11-13)

主引用文献

Wagner, F.R.,Dienemann, C.,Wang, H.,Stutzer, A.,Tegunov, D.,Urlaub, H.,Cramer, P.
Structure of SWI/SNF chromatin remodeller RSC bound to a nucleosome.
Nature, 579:448-451, 2020

PubMed Abstract: Chromatin-remodelling complexes of the SWI/SNF family function in the formation of nucleosome-depleted, transcriptionally active promoter regions (NDRs). In the yeast Saccharomyces cerevisiae, the essential SWI/SNF complex RSC contains 16 subunits, including the ATP-dependent DNA translocase Sth1. RSC removes nucleosomes from promoter regions and positions the specialized +1 and -1 nucleosomes that flank NDRs. Here we present the cryo-electron microscopy structure of RSC in complex with a nucleosome substrate. The structure reveals that RSC forms five protein modules and suggests key features of the remodelling mechanism. The body module serves as a scaffold for the four flexible modules that we call DNA-interacting, ATPase, arm and actin-related protein (ARP) modules. The DNA-interacting module binds extra-nucleosomal DNA and is involved in the recognition of promoter DNA elements that influence RSC functionality. The ATPase and arm modules sandwich the nucleosome disc with the Snf2 ATP-coupling (SnAC) domain and the finger helix, respectively. The translocase motor of the ATPase module engages with the edge of the nucleosome at superhelical location +2. The mobile ARP module may modulate translocase-nucleosome interactions to regulate RSC activity. The RSC-nucleosome structure provides a basis for understanding NDR formation and the structure and function of human SWI/SNF complexes that are frequently mutated in cancer.

PubMed: 32188943
DOI: 10.1038/s41586-020-2088-0

実験手法

ELECTRON MICROSCOPY (15 Å)