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6TBN

Crystal structure of CIAO1-CIAO2B CIA core complex

Summary for 6TBN
Entry DOI10.2210/pdb6tbn/pdb
Related6TBL
DescriptorMIP18 family protein galla-2, Probable cytosolic iron-sulfur protein assembly protein Ciao1, SODIUM ION, ... (4 entities in total)
Functional Keywordsfe-s cluster, fe-s, cia, cia core complex, biosynthetic protein
Biological sourceDrosophila melanogaster (Fruit fly)
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Total number of polymer chains2
Total formula weight55128.39
Authors
Kassube, S.A.,Thoma, N.H. (deposition date: 2019-11-01, release date: 2020-07-29, Last modification date: 2024-11-13)
Primary citationKassube, S.A.,Thoma, N.H.
Structural insights into Fe-S protein biogenesis by the CIA targeting complex.
Nat.Struct.Mol.Biol., 27:735-742, 2020
Cited by
PubMed Abstract: The cytosolic iron-sulfur (Fe-S) assembly (CIA) pathway is required for the insertion of Fe-S clusters into cytosolic and nuclear client proteins, including many DNA replication and repair factors. The molecular mechanisms of client protein recognition and Fe-S cluster transfer remain unknown. Here, we report crystal structures of the CIA targeting complex (CTC), revealing that its CIAO2B subunit is centrally located and bridges CIAO1 and the client adaptor protein MMS19. Cryo-EM reconstructions of human CTC bound either to the DNA replication factor primase or to the DNA helicase DNA2, combined with biochemical, biophysical and yeast complementation assays, reveal an evolutionarily conserved, bipartite client recognition mode facilitated by CIAO1 and the structural flexibility of the MMS19 subunit. Unexpectedly, the primase Fe-S cluster is located ~70 Å away from the CTC reactive cysteine, implicating conformational dynamics of the CTC or additional maturation factors in the mechanism of Fe-S cluster transfer.
PubMed: 32632277
DOI: 10.1038/s41594-020-0454-0
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2 Å)
Structure validation

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数据于2025-06-25公开中

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