6TA1

Fatty acid synthase of S. cerevisiae

これはPDB形式変換不可エントリーです。

6TA1 の概要

• エントリーDOI: 10.2210/pdb6ta1/pdb
• EMDBエントリー: 10420
• 分子名称: Fatty acid synthase subunit alpha, Fatty acid synthase subunit beta, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, ... (4 entities in total)
• 機能のキーワード: megasynthase, complex, biosynthetic protein
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 2624439.28

構造登録者

Vonck, J.,D'Imprima, E.,Joppe, M.,Grininger, M. (登録日: 2019-10-29, 公開日: 2019-11-06, 最終更新日: 2024-11-20)

主引用文献

Joppe, M.,D'Imprima, E.,Salustros, N.,Paithankar, K.S.,Vonck, J.,Grininger, M.,Kuhlbrandt, W.
The resolution revolution in cryoEM requires high-quality sample preparation: a rapid pipeline to a high-resolution map of yeast fatty acid synthase.
Iucrj, 7:220-227, 2020

PubMed Abstract: Single-particle electron cryo-microscopy (cryoEM) has undergone a 'resolution revolution' that makes it possible to characterize megadalton (MDa) complexes at atomic resolution without crystals. To fully exploit the new opportunities in molecular microscopy, new procedures for the cloning, expression and purification of macromolecular complexes need to be explored. Macromolecular assemblies are often unstable, and invasive construct design or inadequate purification conditions and sample-preparation methods can result in disassembly or denaturation. The structure of the 2.6 MDa yeast fatty acid synthase (FAS) has been studied by electron microscopy since the 1960s. Here, a new, streamlined protocol for the rapid production of purified yeast FAS for structure determination by high-resolution cryoEM is reported. Together with a companion protocol for preparing cryoEM specimens on a hydrophilized graphene layer, the new protocol yielded a 3.1 Å resolution map of yeast FAS from 15 000 automatically picked particles within a day. The high map quality enabled a complete atomic model of an intact fungal FAS to be built.

PubMed: 32148850
DOI: 10.1107/S2052252519017366

実験手法

ELECTRON MICROSCOPY (3.1 Å)