6T8F

Crystal structure of mutant xylose isomerase (V270A/A273G) from Piromyces E2 grown in yeast, in complex with xylose

6T8F の概要

• エントリーDOI: 10.2210/pdb6t8f/pdb
• 関連するPDBエントリー: 6T8E
• 分子名称: Xylose isomerase, CALCIUM ION, D-xylose, ... (7 entities in total)
• 機能のキーワード: isomerase, tim-barrel
• 由来する生物種: Piromyces sp. (strain E2)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 201265.72

構造登録者

Rozeboom, H.J.,Janssen, D.B. (登録日: 2019-10-24, 公開日: 2020-01-29, 最終更新日: 2024-01-24)

主引用文献

Lee, M.,Rozeboom, H.J.,Keuning, E.,de Waal, P.,Janssen, D.B.
Structure-based directed evolution improves S. cerevisiae growth on xylose by influencing in vivo enzyme performance.
Biotechnol Biofuels, 13:5-5, 2020

PubMed Abstract: Efficient bioethanol production from hemicellulose feedstocks by requires xylose utilization. Whereas does not metabolize xylose, engineered strains that express xylose isomerase can metabolize xylose by converting it to xylulose. For this, the type II xylose isomerase from (PirXI) is used but the in vivo activity is rather low and very high levels of the enzyme are needed for xylose metabolism. In this study, we explore the use of protein engineering and in vivo selection to improve the performance of PirXI. Recently solved crystal structures were used to focus mutagenesis efforts.

PubMed: 31938040
DOI: 10.1186/s13068-019-1643-0

実験手法

X-RAY DIFFRACTION (2 Å)