6T6C

Complex with chitin oligomer of C-type lysozyme from the upper gastrointestinal tract of Opisthocomus hoatzin

6T6C の概要

• エントリーDOI: 10.2210/pdb6t6c/pdb
• 分子名称: Lysozyme C, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: c-type lysoyme, glycoside hydrolase, hydrolase
• 由来する生物種: Opisthocomus hoazin (Hoatzin)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15381.45

構造登録者

Taylor, E.J.,Skjot, M.,Skov, L.K.,Klausen, M.,De Maria, L.,Gippert, G.P.,Turkenburg, J.P.,Davies, G.J.,Wilson, K.S. (登録日: 2019-10-18, 公開日: 2019-11-20, 最終更新日: 2024-11-13)

主引用文献

Taylor, E.J.,Skjot, M.,Skov, L.K.,Klausen, M.,De Maria, L.,Gippert, G.P.,Turkenburg, J.P.,Davies, G.J.,Wilson, K.S.
The C-Type Lysozyme from the upper Gastrointestinal Tract of Opisthocomus hoatzin, the Stinkbird.
Int J Mol Sci, 20:-, 2019

PubMed Abstract: Muramidases/lysozymes are important bio-molecules, which cleave the glycan backbone in the peptidoglycan polymer found in bacterial cell walls. The glycoside hydrolase (GH) family 22 C-type lysozyme, from the folivorous bird (stinkbird), was expressed in , and a set of variants was produced. All variants were enzymatically active, including those designed to probe key differences between the Hoatzin enzyme and Hen Egg White lysozyme. Four variants showed improved thermostability at pH 4.7, compared to the wild type. The X-ray structure of the enzyme was determined in the apo form and in complex with chitin oligomers. Bioinformatic analysis of avian GH22 amino acid sequences showed that they separate out into three distinct subgroups (chicken-like birds, sea birds and other birds). The Hoatzin is found in the "other birds" group and we propose that this represents a new cluster of avian upper-gut enzymes.

PubMed: 31698762
DOI: 10.3390/ijms20225531

実験手法

X-RAY DIFFRACTION (1.25 Å)