6T5S

Apo form of C-type lysozyme from the upper gastrointestinal tract of Opisthocomus hoatzin

Summary for 6T5S

• Entry DOI: 10.2210/pdb6t5s/pdb
• Descriptor: Lysozyme C, GLYCEROL, CHLORIDE ION, ... (4 entities in total)
• Functional Keywords: c-type lysoyme, glycoside hydrolase, hydrolase
• Biological source: Opisthocomus hoazin (Hoatzin)
• Total number of polymer chains: 1
• Total formula weight: 14520.78

Authors

Taylor, E.J.,Skjot, M.,Skov, L.K.,Klausen, M.,De Maria, L.,Gippert, G.P.,Turkenburg, J.P.,Davies, G.J.,Wilson, K.S. (deposition date: 2019-10-17, release date: 2019-11-20, Last modification date: 2024-11-06)

Primary citation

Taylor, E.J.,Skjot, M.,Skov, L.K.,Klausen, M.,De Maria, L.,Gippert, G.P.,Turkenburg, J.P.,Davies, G.J.,Wilson, K.S.
The C-Type Lysozyme from the upper Gastrointestinal Tract of Opisthocomus hoatzin, the Stinkbird.
Int J Mol Sci, 20:-, 2019

PubMed Abstract: Muramidases/lysozymes are important bio-molecules, which cleave the glycan backbone in the peptidoglycan polymer found in bacterial cell walls. The glycoside hydrolase (GH) family 22 C-type lysozyme, from the folivorous bird (stinkbird), was expressed in , and a set of variants was produced. All variants were enzymatically active, including those designed to probe key differences between the Hoatzin enzyme and Hen Egg White lysozyme. Four variants showed improved thermostability at pH 4.7, compared to the wild type. The X-ray structure of the enzyme was determined in the apo form and in complex with chitin oligomers. Bioinformatic analysis of avian GH22 amino acid sequences showed that they separate out into three distinct subgroups (chicken-like birds, sea birds and other birds). The Hoatzin is found in the "other birds" group and we propose that this represents a new cluster of avian upper-gut enzymes.

PubMed: 31698762
DOI: 10.3390/ijms20225531

Experimental method

X-RAY DIFFRACTION (1.5 Å)