6T59

Structure of rabbit 80S ribosome translating beta-tubulin in complex with tetratricopeptide protein 5 and nascent chain-associated complex

これはPDB形式変換不可エントリーです。

6T59 の概要

• エントリーDOI: 10.2210/pdb6t59/pdb
• EMDBエントリー: 10380
• 分子名称: Ribosomal protein L8, Ribosomal protein L11, eL13, ... (55 entities in total)
• 機能のキーワード: tubulin, nascent chain-associated complex, ribosome-nascent chain, tetratricopeptide protein 5, autoregulation, ribosome
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 53
• 化学式量合計: 2262178.98

構造登録者

Lin, Z.,Gasic, I.,Chandrasekaran, V.,Peters, N.,Shao, S.,Ramakrishnan, V.,Mitchison, T.J.,Hegde, R.S. (登録日: 2019-10-15, 公開日: 2019-11-27, 最終更新日: 2024-11-13)

主引用文献

Lin, Z.,Gasic, I.,Chandrasekaran, V.,Peters, N.,Shao, S.,Mitchison, T.J.,Hegde, R.S.
TTC5 mediates autoregulation of tubulin via mRNA degradation.
Science, 367:100-104, 2020

PubMed Abstract: Tubulins play crucial roles in cell division, intracellular traffic, and cell shape. Tubulin concentration is autoregulated by feedback control of messenger RNA (mRNA) degradation via an unknown mechanism. We identified tetratricopeptide protein 5 (TTC5) as a tubulin-specific ribosome-associating factor that triggers cotranslational degradation of tubulin mRNAs in response to excess soluble tubulin. Structural analysis revealed that TTC5 binds near the ribosome exit tunnel and engages the amino terminus of nascent tubulins. TTC5 mutants incapable of ribosome or nascent tubulin interaction abolished tubulin autoregulation and showed chromosome segregation defects during mitosis. Our findings show how a subset of mRNAs can be targeted for coordinated degradation by a specificity factor that recognizes the nascent polypeptides they encode.

PubMed: 31727855
DOI: 10.1126/science.aaz4352

実験手法

ELECTRON MICROSCOPY (3.11 Å)