6T36

Crystal structure of the PTPN3 PDZ domain bound to the HBV core protein C-terminal peptide

6T36 の概要

• エントリーDOI: 10.2210/pdb6t36/pdb
• 分子名称: Tyrosine-protein phosphatase non-receptor type 3, Capsid protein, BROMIDE ION, ... (4 entities in total)
• 機能のキーワード: pdz domain, hbv core, hbv capsid, hepatitis b virus, ptpn3, pdz-binding motif, hydrolase
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 128597.98

構造登録者

Genera, M.,Mechaly, A.,Haouz, A.,Caillet-Saguy, C. (登録日: 2019-10-10, 公開日: 2021-01-20, 最終更新日: 2024-01-24)

主引用文献

Genera, M.,Quioc-Salomon, B.,Nourisson, A.,Colcombet-Cazenave, B.,Haouz, A.,Mechaly, A.,Matondo, M.,Duchateau, M.,Konig, A.,Windisch, M.P.,Neuveut, C.,Wolff, N.,Caillet-Saguy, C.
Molecular basis of the interaction of the human tyrosine phosphatase PTPN3 with the hepatitis B virus core protein.
Sci Rep, 11:944-944, 2021

PubMed Abstract: Interactions between the hepatitis B virus core protein (HBc) and host cell proteins are poorly understood, although they may be essential for the propagation of the virus and its pathogenicity. HBc has a C-terminal PDZ (PSD-95, Dlg1, ZO-1)-binding motif (PBM) that is responsible for interactions with host PDZ domain-containing proteins. In this work, we focused on the human protein tyrosine phosphatase non-receptor type 3 (PTPN3) and its interaction with HBc. We solved the crystal structure of the PDZ domain of PTPN3 in complex with the PBM of HBc, revealing a network of interactions specific to class I PDZ domains despite the presence of a C-terminal cysteine in this atypical PBM. We further showed that PTPN3 binds the HBc protein within capsids or as a homodimer. We demonstrate that overexpression of PTPN3 significantly affects HBV infection in HepG2 NTCP cells. Finally, we performed proteomics studies on both sides by pull-down assays and screening of a human PDZ domain library. We identified a pool of human PBM-containing proteins that might interact with PTPN3 in cells and that could be in competition with the HBc PBM during infection, and we also identified potential cellular partners of HBc through PDZ-PBM interactions. This study opens up many avenues of future investigations into the pathophysiology of HBV.

PubMed: 33441627
DOI: 10.1038/s41598-020-79580-9

実験手法

X-RAY DIFFRACTION (1.86 Å)