6SXR

E221Q mutant of GH54 a-l-arabinofuranosidase soaked with 4-nitrophenyl a-l-arabinofuranoside

6SXR の概要

• エントリーDOI: 10.2210/pdb6sxr/pdb
• 分子名称: Alpha-L-arabinofuranosidase B, PENTAETHYLENE GLYCOL, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (11 entities in total)
• 機能のキーワード: michaelis complex, arabinofuranosidase, gh54, aspergillus, hydrolase
• 由来する生物種: Aspergillus kawachii IFO 4308
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 55030.68

構造登録者

McGregor, N.G.S.,Davies, G.J.,Nin-Hill, A.,Rovira, C. (登録日: 2019-09-26, 公開日: 2020-02-26, 最終更新日: 2024-10-23)

主引用文献

McGregor, N.G.S.,Artola, M.,Nin-Hill, A.,Linzel, D.,Haon, M.,Reijngoud, J.,Ram, A.,Rosso, M.N.,van der Marel, G.A.,Codee, J.D.C.,van Wezel, G.P.,Berrin, J.G.,Rovira, C.,Overkleeft, H.S.,Davies, G.J.
Rational Design of Mechanism-Based Inhibitors and Activity-Based Probes for the Identification of Retaining alpha-l-Arabinofuranosidases.
J.Am.Chem.Soc., 142:4648-4662, 2020

PubMed Abstract: Identifying and characterizing the enzymes responsible for an observed activity within a complex eukaryotic catabolic system remains one of the most significant challenges in the study of biomass-degrading systems. The debranching of both complex hemicellulosic and pectinaceous polysaccharides requires the production of α-l-arabinofuranosidases among a wide variety of coexpressed carbohydrate-active enzymes. To selectively detect and identify α-l-arabinofuranosidases produced by fungi grown on complex biomass, potential covalent inhibitors and probes which mimic α-l-arabinofuranosides were sought. The conformational free energy landscapes of free α-l-arabinofuranose and several rationally designed covalent α-l-arabinofuranosidase inhibitors were analyzed. A synthetic route to these inhibitors was subsequently developed based on a key Wittig-Still rearrangement. Through a combination of kinetic measurements, intact mass spectrometry, and structural experiments, the designed inhibitors were shown to efficiently label the catalytic nucleophiles of retaining GH51 and GH54 α-l-arabinofuranosidases. Activity-based probes elaborated from an inhibitor with an aziridine warhead were applied to the identification and characterization of α-l-arabinofuranosidases within the secretome of grown on arabinan. This method was extended to the detection and identification of α-l-arabinofuranosidases produced by eight biomass-degrading basidiomycete fungi grown on complex biomass. The broad applicability of the cyclophellitol-derived activity-based probes and inhibitors presented here make them a valuable new tool in the characterization of complex eukaryotic carbohydrate-degrading systems and in the high-throughput discovery of α-l-arabinofuranosidases.

PubMed: 32053363
DOI: 10.1021/jacs.9b11351

実験手法

X-RAY DIFFRACTION (1.64 Å)