6SUP

Crystal Structure of TcdB2-TccC3-Cdc42

6SUP の概要

• エントリーDOI: 10.2210/pdb6sup/pdb
• 分子名称: TcdB2,TccC3,Cell division control protein 42 homolog, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: toxins, tc toxins, toxin
• 由来する生物種: Photorhabdus luminescens; 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 240903.46

構造登録者

Roderer, D.,Schubert, E.,Sitsel, O.,Raunser, S. (登録日: 2019-09-16, 公開日: 2019-12-04, 最終更新日: 2024-11-20)

主引用文献

Roderer, D.,Schubert, E.,Sitsel, O.,Raunser, S.
Towards the application of Tc toxins as a universal protein translocation system.
Nat Commun, 10:5263-5263, 2019

PubMed Abstract: Tc toxins are bacterial protein complexes that inject cytotoxic enzymes into target cells using a syringe-like mechanism. Tc toxins are composed of a membrane translocator and a cocoon that encapsulates a toxic enzyme. The toxic enzyme varies between Tc toxins from different species and is not conserved. Here, we investigate whether the toxic enzyme can be replaced by other small proteins of different origin and properties, namely Cdc42, herpes simplex virus ICP47, Arabidopsis thaliana iLOV, Escherichia coli DHFR, Ras-binding domain of CRAF kinase, and TEV protease. Using a combination of electron microscopy, X-ray crystallography and in vitro translocation assays, we demonstrate that it is possible to turn Tc toxins into customizable molecular syringes for delivering proteins of interest across membranes. We also infer the guidelines that protein cargos must obey in terms of size, charge, and fold in order to apply Tc toxins as a universal protein translocation system.

PubMed: 31748551
DOI: 10.1038/s41467-019-13253-8

実験手法

X-RAY DIFFRACTION (2 Å)