6STA
Crystal structure of the strawberry pathogenesis-related 10 (PR-10) Fra a 1.02 protein, E46A D48A mutant
6STA の概要
エントリーDOI | 10.2210/pdb6sta/pdb |
関連するPDBエントリー | 5AMW |
分子名称 | Major strawberry allergen Fra a 1-2 (2 entities in total) |
機能のキーワード | fragaria x ananassa, fra a 1, allergen, fruit, ripening, pr-10 |
由来する生物種 | Fragaria ananassa (Strawberry) |
タンパク質・核酸の鎖数 | 2 |
化学式量合計 | 35235.96 |
構造登録者 | Orozco-Navarrete, B.,Kaczmarska, Z.,Dupeux, F.,Pott, D.,Diaz Perales, A.,Casanal, A.,Marquez, J.A.,Valpuesta, V.,Merchante, C. (登録日: 2019-09-10, 公開日: 2019-12-18, 最終更新日: 2024-01-24) |
主引用文献 | Orozco-Navarrete, B.,Kaczmarska, Z.,Dupeux, F.,Garrido-Arandia, M.,Pott, D.,Perales, A.D.,Casanal, A.,Marquez, J.A.,Valpuesta, V.,Merchante, C. Structural Bases for the Allergenicity of Fra a 1.02 in Strawberry Fruits. J.Agric.Food Chem., 68:10951-10961, 2020 Cited by PubMed Abstract: Although strawberries are highly appreciated fruits, their intake can induce allergic reactions in atopic patients. These reactions can be due to the patient's previous sensitization to the major birch pollen allergen Bet v 1, by which IgE generated in response to Bet v 1 cross-reacts with the structurally related strawberry Fra a 1 protein family. Fra a 1.02 is the most expressed paralog in ripe strawberries and is highly allergenic. To better understand the molecular mechanisms regulating this allergic response, we have determined the three-dimensional structure of Fra a 1.02 and four site-directed mutants that were designed based on their positions in potential epitopes. Fra a 1.02 and mutants conform to the START fold. We show that the cross-reactivity of all the mutant variants to IgE from patients allergic to Bet v 1 was significantly reduced without altering the conserved structural fold, so that they could potentially be used as hypoallergenic Fra a 1 variants for the generation of vaccines against strawberry allergy in atopic patients. PubMed: 31774998DOI: 10.1021/acs.jafc.9b05714 主引用文献が同じPDBエントリー |
実験手法 | X-RAY DIFFRACTION (2.19 Å) |
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