6SSV

The structure of serpin from Schistosoma mansoni

6SSV の概要

• エントリーDOI: 10.2210/pdb6ssv/pdb
• 分子名称: Serpin, putative (1 entity in total)
• 機能のキーワード: serine protease inhibitor, antigen, allergen
• 由来する生物種: Schistosoma mansoni (Blood fluke)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 43935.34

構造登録者

De Benedetti, S.,Gourlay, L. (登録日: 2019-09-09, 公開日: 2020-09-30, 最終更新日: 2024-01-24)

主引用文献

De Benedetti, S.,Di Pisa, F.,Fassi, E.M.A.,Cretich, M.,Musico, A.,Frigerio, R.,Mussida, A.,Bombaci, M.,Grifantini, R.,Colombo, G.,Bolognesi, M.,Grande, R.,Zanchetta, N.,Gismondo, M.R.,Mileto, D.,Mancon, A.,Gourlay, L.J.
Structure, Immunoreactivity, and In Silico Epitope Determination of SmSPI S. mansoni Serpin for Immunodiagnostic Application.
Vaccines (Basel), 9:-, 2021

PubMed Abstract: The human parasitic disease Schistosomiasis is caused by the trematode flatworm that infects freshwaters in tropical regions of the world, particularly in Sub-Saharan Africa, South America, and the Far-East. It has also been observed as an emerging disease in Europe, due to increased immigration. In addition to improved therapeutic strategies, it is imperative to develop novel, rapid, and sensitive diagnostic tests that can detect the parasite, allowing timely treatment. Present diagnosis is difficult and involves microscopy-based detection of Schistosoma eggs in the feces. In this context, we present the 3.22 Å resolution crystal structure of the circulating antigen Serine protease inhibitor from (SmSPI), and we describe it as a potential serodiagnostic marker. Moreover, we identify three potential immunoreactive epitopes using in silico-based epitope mapping methods. Here, we confirm effective immune sera reactivity of the recombinant antigen, suggesting the further investigation of the protein and/or its predicted epitopes as serodiagnostic Schistosomiasis biomarkers.

PubMed: 33915716
DOI: 10.3390/vaccines9040322

実験手法

X-RAY DIFFRACTION (3.22 Å)