6SKE

Teneurin 2 in complex with Latrophilin 2 Lec domain

6SKE の概要

• エントリーDOI: 10.2210/pdb6ske/pdb
• 分子名称: Teneurin-2, Adhesion G protein-coupled receptor L2, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: teneurin, latrophilin, odz, signalling, adhesion, repulsion, synapse, neurons, cell migration, adgrl, signaling protein
• 由来する生物種: Gallus gallus (Chicken); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 427777.51

構造登録者

Shahin, M.,Jackson, V.A.,Carrasquero, M.,Lowe, E.,Seiradake, E. (登録日: 2019-08-15, 公開日: 2020-02-12, 最終更新日: 2024-11-20)

主引用文献

Del Toro, D.,Carrasquero-Ordaz, M.A.,Chu, A.,Ruff, T.,Shahin, M.,Jackson, V.A.,Chavent, M.,Berbeira-Santana, M.,Seyit-Bremer, G.,Brignani, S.,Kaufmann, R.,Lowe, E.,Klein, R.,Seiradake, E.
Structural Basis of Teneurin-Latrophilin Interaction in Repulsive Guidance of Migrating Neurons.
Cell, 180:323-339.e19, 2020

PubMed Abstract: Teneurins are ancient metazoan cell adhesion receptors that control brain development and neuronal wiring in higher animals. The extracellular C terminus binds the adhesion GPCR Latrophilin, forming a trans-cellular complex with synaptogenic functions. However, Teneurins, Latrophilins, and FLRT proteins are also expressed during murine cortical cell migration at earlier developmental stages. Here, we present crystal structures of Teneurin-Latrophilin complexes that reveal how the lectin and olfactomedin domains of Latrophilin bind across a spiraling beta-barrel domain of Teneurin, the YD shell. We couple structure-based protein engineering to biophysical analysis, cell migration assays, and in utero electroporation experiments to probe the importance of the interaction in cortical neuron migration. We show that binding of Latrophilins to Teneurins and FLRTs directs the migration of neurons using a contact repulsion-dependent mechanism. The effect is observed with cell bodies and small neurites rather than their processes. The results exemplify how a structure-encoded synaptogenic protein complex is also used for repulsive cell guidance.

PubMed: 31928845
DOI: 10.1016/j.cell.2019.12.014

実験手法

X-RAY DIFFRACTION (3.62 Å)