6SJB

Cryo-EM structure of the RecBCD Chi recognised complex

6SJB の概要

• エントリーDOI: 10.2210/pdb6sjb/pdb
• EMDBエントリー: 10214
• 分子名称: RecBCD enzyme subunit RecB, RecBCD enzyme subunit RecC, RecBCD enzyme subunit RecD, ... (4 entities in total)
• 機能のキーワード: dna repair, homologous recombination, atp hydrolysis, helicase, nuclease, translocation, hydrolase
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 356247.82

構造登録者

Cheng, K.,Wilkinson, M.,Wigley, D.B. (登録日: 2019-08-13, 公開日: 2020-01-01, 最終更新日: 2024-05-22)

主引用文献

Cheng, K.,Wilkinson, M.,Chaban, Y.,Wigley, D.B.
A conformational switch in response to Chi converts RecBCD from phage destruction to DNA repair.
Nat.Struct.Mol.Biol., 27:71-77, 2020

PubMed Abstract: The RecBCD complex plays key roles in phage DNA degradation, CRISPR array acquisition (adaptation) and host DNA repair. The switch between these roles is regulated by a DNA sequence called Chi. We report cryo-EM structures of the Escherichia coli RecBCD complex bound to several different DNA forks containing a Chi sequence, including one in which Chi is recognized and others in which it is not. The Chi-recognized structure shows conformational changes in regions of the protein that contact Chi and reveals a tortuous path taken by the DNA. Sequence specificity arises from interactions with both the RecC subunit and the sequence itself. These structures provide molecular details for how Chi is recognized and insights into the changes that occur in response to Chi binding that switch RecBCD from bacteriophage destruction and CRISPR spacer acquisition to constructive host DNA repair.

PubMed: 31907455
DOI: 10.1038/s41594-019-0355-2

実験手法

ELECTRON MICROSCOPY (3.7 Å)